6-dR6G [6-Carboxy-4,7-dichlororhodamine 6G]

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Physical properties

Molecular weight	628.59
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

628.59

6-dR6G (6-Carboxy-4,7-dichlororhodamine 6G) is one of the four critical 4,7-dichloro-rhodamine dyes used as the BigDye terminators for DNA sequencing in combination with dR110, dTMR and dROX. ABI PRISM® BigDye™ Terminator Cycle Sequencing is the classic method for DNA sequencing.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-dR6G [6-Carboxy-4,7-dichlororhodamine 6G] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	159.086 µL	795.431 µL	1.591 mL	7.954 mL	15.909 mL
5 mM	31.817 µL	159.086 µL	318.172 µL	1.591 mL	3.182 mL
10 mM	15.909 µL	79.543 µL	159.086 µL	795.431 µL	1.591 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-CR110 [6-Carboxyrhodamine 110] Single isomer	499	525	84000	0.091
6-CR6G [6-Carboxyrhodamine 6G] Single isomer	522	546	94000	0.214
6-TAMRA [6-Carboxytetramethylrhodamine] CAS 91809-67-5	552	578	90000	0.178
6-ROX [6-Carboxy-X-rhodamine] CAS 194785-18-7	578	604	82000	0.168
6-TRITC [Tetramethylrhodamine-6-isothiocyanate] CAS 80724-20-5	544	570	100000	0.34
6-FAM [6-Carboxyfluorescein] CAS 3301-79-9	493	517	83000	0.178
6-DTAF [6-(4,6-Dichlorotriazinyl)aminofluorescein] CAS 118357-32-7	498	517	80000¹	0.35

Images

Figure 1. Chemical structure for 6-dR6G [6-Carboxy-4,7-dichlororhodamine 6G]

References

View all 6 references: Citation Explorer

Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing
Authors: Tan, L.; Liu, Y.; Yang, Q.; Li, X.; Wu, X. Y.; Gong, B.; Shen, Y. M.; Shao, Z.
Journal: Chem Commun (Camb) (2016): 954-7

Synthesis of novel tyrosinyl FRET cassettes, terminators, and their potential use in DNA sequencing
Authors: Sudhakar Rao, T.; Zhang, W.; Xiao, H.; Flick, P.; Kumar, S.; Nampalli, S.
Journal: Nucleosides Nucleotides Nucleic Acids (2003): 1443-5

Improved DNA sequencing accuracy and detection of heterozygous alleles using manganese citrate and different fluorescent dye terminators
Authors: Korch, C.; Drabkin, H.
Journal: Genome Res (1999): 588-95

Design strategies and performance of custom DNA sequencing primers
Authors: Buck, G. A.; Fox, J. W.; Gunthorpe, M.; Hager, K. M.; Naeve, C. W.; Pon, R. T.; Adams, P. S.; Rush, J.
Journal: Biotechniques (1999): 528-36

Peak height pattern in dichloro-rhodamine and energy transfer dye terminator sequencing
Authors: Zakeri, H.; Amparo, G.; Chen, S. M.; Spurgeon, S.; Kwok, P. Y.
Journal: Biotechniques (1998): 406-10, 412-4

New dye-labeled terminators for improved DNA sequencing patterns
Authors: Rosenblum, B. B.; Lee, L. G.; Spurgeon, S. L.; Khan, S. H.; Menchen, S. M.; Heiner, C. R.; Chen, S. M.
Journal: Nucleic Acids Res (1997): 4500-4