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6-TAMRA [6-Carboxytetramethylrhodamine] *CAS 91809-67-5*

6-TAMRA is the purified single isomer of 5(6)-TAMRA. It is widely used to label nucleotides and nucleic acids. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates. Currently AAT Bioquest is using a proprietary process to synthesize isomer-free 6-TAMRA without the conventional chromatographic purification.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TAMRA [6-Carboxytetramethylrhodamine] *CAS 91809-67-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM232.315 µL1.162 mL2.323 mL11.616 mL23.232 mL
5 mM46.463 µL232.315 µL464.63 µL2.323 mL4.646 mL
10 mM23.232 µL116.158 µL232.315 µL1.162 mL2.323 mL

Molarity calculator

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Spectrum

Citations

View all 3 citations: Citation Explorer
Design and Characterization of Two Bifunctional Cryptophane A-Based Host Molecules for Xenon Magnetic Resonance Imaging Applications
Authors: Rossella, Federica and Rose, Honor May and Witte, Christopher and Jayapaul, Jabadurai and Schröder, Leif
Journal: ChemPlusChem (2014): 1463--1471
Human erythrocytes as drug carriers: loading efficiency and side effects of hypotonic dialysis, chlorpromazine treatment and fusion with liposomes
Authors: Favretto, ME and Cluitmans, JCA and Bosman, GJCGM and Brock, R
Journal: Journal of Controlled Release (2013): 343--351
Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

References

View all 96 references: Citation Explorer
Fluorescence anisotropy and FRET studies of G-quadruplex formation in presence of different cations
Authors: Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 835
G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730
A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway
Authors: Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.
Journal: Chembiochem (2006): 1428
Electrostatic-gated transport in chemically modified glass nanopore electrodes
Authors: Wang G, Zhang B, Wayment JR, Harris JM, White HS.
Journal: J Am Chem Soc (2006): 7679
Metal-enhanced fluorescence-based RNA sensing
Authors: Aslan K, Huang J, Wilson GM, Geddes CD.
Journal: J Am Chem Soc (2006): 4206
Page updated on October 12, 2024

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Physical properties

Molecular weight

430.45

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm -1 M -1)

90000

Excitation (nm)

552

Emission (nm)

578

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

91809-67-5