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Bodi Fluor™ 576/589 NHS Ester (equivalent to BODIPY™ 576/589 NHS Ester)

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Physical properties
Molecular weight426.19
SolventDMSO
Spectral properties
Excitation (nm)573
Emission (nm)592
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
201998-61-0
Molecular weight
426.19
Excitation (nm)
573
Emission (nm)
592
Bodi Fluor™ 576/589 is chemically the same molecule to BODIPY™ 576/589 dye. It is a bright red fluorescent dye with high cell-permeability. It has a high extinction coefficient and fluorescence quantum yield. Its fluorescence is relatively insensitive to solvent polarity and pH change. In contrast to the highly water-soluble fluorophores such as iFluor® and Alexa Fluor® 488 dyes, Bodi Fluor dyes have unique hydrophobic properties ideal for staining lipids, membranes, and other lipophilic compounds. Bodi Fluor 576/589 dye has a relatively long excited-state lifetime (typically 5 nanoseconds or longer), which is useful for fluorescence polarization-based assays and a large two-photon cross-section for multiphoton excitation. Bodi Fluor™ 576/589 NHS Ester can be readily used for labeling any amino-containing biomolecules such as proteins, peptides and amino-modified oligos.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Bodi Fluor™ 576/589 NHS Ester (equivalent to BODIPY™ 576/589 NHS Ester) to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM234.637 µL1.173 mL2.346 mL11.732 mL23.464 mL
5 mM46.927 µL234.637 µL469.274 µL2.346 mL4.693 mL
10 mM23.464 µL117.319 µL234.637 µL1.173 mL2.346 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)573
Emission (nm)592

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References


View all 49 references: Citation Explorer
Living Supramolecular Polymerization of an Aza-BODIPY Dye Controlled by a Hydrogen-Bond-Accepting Triazole Unit Introduced by Click Chemistry.
Authors: Wang, Houchen and Zhang, Yongjie and Chen, Yuanfang and Pan, Hongfei and Ren, Xiangkui and Chen, Zhijian
Journal: Angewandte Chemie (International ed. in English) (2020): 5185-5192
Targeting EGFR Overexpression at the Surface of Colorectal Cancer Cells by Exploiting Amidated BODIPY-Peptide Conjugates.
Authors: Williams, Tyrslai M and Zhou, Zehua and Singh, Sitanshu S and Sibrian-Vazquez, Martha and Jois, Seetharama D and Henriques Vicente, Maria da Graça
Journal: Photochemistry and photobiology (2020): 581-595
Amphiphilic BODIPY dye aggregates in polymeric micelles for wavelength-dependent photo-induced cancer therapy.
Authors: Yan, Ziling and Wang, Mengya and Shi, Mengke and He, Yang and Zhang, Yi and Qiu, Shihong and Yang, Hong and Chen, Huabing and He, Hui and Guo, Zhengqing
Journal: Journal of materials chemistry. B (2020)
Absorption properties of a BODIPY-curved graphene nanoflake system: A theoretical investigation.
Authors: Petrushenko, I K and Petrushenko, K B
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 117465
Two π-Electrons Make the Difference: From BODIPY to BODIIM Switchable Fluorescent Dyes.
Authors: Dolati, Hadi and Haufe, Lisa C and Denker, Lars and Lorbach, Andreas and Grotjahn, Robin and Hörner, Gerald and Frank, René
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 1422-1428
Exploring the Relationship between BODIPY Structure and Spectroscopic Properties to Design Fluorophores for Bioimaging.
Authors: Donnelly, Joanna L and Offenbartl-Stiegert, Daniel and Marín-Beloqui, José M and Rizzello, Loris and Battaglia, Guiseppe and Clarke, Tracey M and Howorka, Stefan and Wilden, Jonathan D
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 863-872
Microalgae Lipid Staining with Fluorescent BODIPY Dye.
Authors: Koreivienė, Judita
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 47-53
Rational design of a boron-dipyrromethene-based fluorescent probe for detecting Pd2+ sensitively and selectively in aqueous media.
Authors: Li, Yannan and Yang, Li and Du, Mengqi and Chang, Guanjun
Journal: The Analyst (2019): 1260-1264
Mechanoresponsive Behavior of a Polymer-Embedded Red-Light Emitting Rotaxane Mechanophore.
Authors: Muramatsu, Tatsuya and Sagara, Yoshimitsu and Traeger, Hanna and Tamaoki, Nobuyuki and Weder, Christoph
Journal: ACS applied materials & interfaces (2019): 24571-24576
A near-infrared I emissive dye: toward the application of saturable absorber and multiphoton fluorescence microscopy in the deep-tissue imaging window.
Authors: Ren, Can and Deng, Xiangquan and Hu, Wenbo and Li, Junzi and Miao, Xiaofei and Xiao, Shuyu and Liu, Hongji and Fan, Quli and Wang, Ke and He, Tingchao
Journal: Chemical communications (Cambridge, England) (2019): 5111-5114