AAT Bioquest


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Physical properties
Molecular weight598.30
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
7-Deaza-7-Propargylamino-3'-azidomethyl-dATP is a key building block for preparing fluorescent conjugates that are used in the next generation sequencing (NGS). NGS uses a similar chain termination method to the earlier Sanger sequencing, but NGS is carried out by fluorescence-labeled nucleotide analogs acting as reversible terminators of the amplification reaction. NGS relies on the blockade of DNA polymerization that is reversible while the Sanger sequencing uses the irreversible blockade of DNA polymerization by ddNTPs. Another different feature of NGS is that the clonal amplification in vitro to multiply the number of molecules to be sequenced is conducted by means of bridge PCR. In this platform, the fragments are joined to primers immobilized on a solid surface, performing an amplification in situ, generating clusters of DNA with identical molecules. In each cycle, the four nucleotides of reversible termination are simultaneously added and incorporated by the polymerase they complement. These nucleotides are chemically blocked—by substituting the 3′-OH group for a 3′-o-azidomethyl group—to prevent the polymerase from incorporating more than one nucleotide in each cycle. Upon incorporation of a nucleotide, a fluorescence signal is measured in different channels for different bases. Concerning the next cycle, the nucleotides that have not been incorporated are washed and the chemical blockade of the 3′ end is removed with TCEP. Once the fluorescence signal is collected, a new cycle begins, repeating this dynamic until the sequencing of each fragment is finished. In summary, the NGS sequencing reaction is carried out in three steps: addition of nucleotides, imaging, and regeneration of 3′-OH by fluorophore cleavage.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of 7-Deaza-7-Propargylamino-3'-azidomethyl-dATP to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM167.14 µL835.701 µL1.671 mL8.357 mL16.714 mL
5 mM33.428 µL167.14 µL334.28 µL1.671 mL3.343 mL
10 mM16.714 µL83.57 µL167.14 µL835.701 µL1.671 mL

Molarity calculator

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View all 4 references: Citation Explorer
Single-tube, highly parallel mutation enrichment in cancer gene panels by use of temperature-tolerant COLD-PCR.
Authors: Castellanos-Rizaldos, Elena and Richardson, Katherine and Lin, Rui and Wu, Grant and Makrigiorgos, Mike G
Journal: Clinical chemistry (2015): 267-77
Transcriptome analysis of Capsicum annuum varieties Mandarin and Blackcluster: assembly, annotation and molecular marker discovery.
Authors: Ahn, Yul-Kyun and Tripathi, Swati and Kim, Jeong-Ho and Cho, Young-Il and Lee, Hye-Eun and Kim, Do-Sun and Woo, Jong-Gyu and Cho, Myeong-Cheoul
Journal: Gene (2014): 494-9
Mutant firefly luciferases with improved specific activity and dATP discrimination constructed by yeast cell surface engineering.
Authors: Fushimi, Tatsuya and Miura, Natsuko and Shintani, Hideya and Tsunoda, Hiroyuki and Kuroda, Kouichi and Ueda, Mitsuyoshi
Journal: Applied microbiology and biotechnology (2013): 4003-11
Termination of DNA synthesis by N6-alkylated, not 3'-O-alkylated, photocleavable 2'-deoxyadenosine triphosphates.
Authors: Wu, Weidong and Stupi, Brian P and Litosh, Vladislav A and Mansouri, Dena and Farley, Demetra and Morris, Sidney and Metzker, Sherry and Metzker, Michael L
Journal: Nucleic acids research (2007): 6339-49