AAT Bioquest


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Physical properties
Molecular weight614.30
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
7-Deaza-7-Propargylamino-3'-azidomethyl-dGTP is a key building block for preparing fluorescent conjugates that are used in the next generation sequencing (NGS). NGS uses a similar chain termination method to the earlier Sanger sequencing, but NGS is carried out by fluorescence-labeled nucleotide analogs acting as reversible terminators of the amplification reaction. NGS relies on the blockade of DNA polymerization that is reversible while the Sanger sequencing uses the irreversible blockade of DNA polymerization by ddNTPs. Another different feature of NGS is that the clonal amplification in vitro to multiply the number of molecules to be sequenced is conducted by means of bridge PCR. In this platform, the fragments are joined to primers immobilized on a solid surface, performing an amplification in situ, generating clusters of DNA with identical molecules. In each cycle, the four nucleotides of reversible termination are simultaneously added and incorporated by the polymerase they complement. These nucleotides are chemically blocked—by substituting the 3′-OH group for a 3′-o-azidomethyl group—to prevent the polymerase from incorporating more than one nucleotide in each cycle. Upon incorporation of a nucleotide, a fluorescence signal is measured in different channels for different bases. Concerning the next cycle, the nucleotides that have not been incorporated are washed and the chemical blockade of the 3′ end is removed with TCEP. Once the fluorescence signal is collected, a new cycle begins, repeating this dynamic until the sequencing of each fragment is finished. In summary, the NGS sequencing reaction is carried out in three steps: addition of nucleotides, imaging, and regeneration of 3′-OH by fluorophore cleavage.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of 7-Deaza-7-Propargylamino-3'-azidomethyl-dGTP to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM162.787 µL813.935 µL1.628 mL8.139 mL16.279 mL
5 mM32.557 µL162.787 µL325.574 µL1.628 mL3.256 mL
10 mM16.279 µL81.393 µL162.787 µL813.935 µL1.628 mL

Molarity calculator

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View all 7 references: Citation Explorer
SAMHD1-deficient fibroblasts from Aicardi-Goutières Syndrome patients can escape senescence and accumulate mutations.
Authors: Franzolin, Elisa and Coletta, Sara and Ferraro, Paola and Pontarin, Giovanna and D'Aronco, Giulia and Stevanoni, Martina and Palumbo, Elisa and Cagnin, Stefano and Bertoldi, Loris and Feltrin, Erika and Valle, Giorgio and Russo, Antonella and Bianchi, Vera and Rampazzo, Chiara
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2020): 631-647
A novel target enrichment strategy in next-generation sequencing through 7-deaza-dGTP-resistant enzymatic digestion.
Authors: Peng, Peng and Xu, Yanjuan and Di Bisceglie, Adrian M and Fan, Xiaofeng
Journal: BMC research notes (2020): 445
Suppressors of dGTP Starvation in Escherichia coli.
Authors: Itsko, Mark and Schaaper, Roel M
Journal: Journal of bacteriology (2017)
Single-tube, highly parallel mutation enrichment in cancer gene panels by use of temperature-tolerant COLD-PCR.
Authors: Castellanos-Rizaldos, Elena and Richardson, Katherine and Lin, Rui and Wu, Grant and Makrigiorgos, Mike G
Journal: Clinical chemistry (2015): 267-77
Transcriptome analysis of Capsicum annuum varieties Mandarin and Blackcluster: assembly, annotation and molecular marker discovery.
Authors: Ahn, Yul-Kyun and Tripathi, Swati and Kim, Jeong-Ho and Cho, Young-Il and Lee, Hye-Eun and Kim, Do-Sun and Woo, Jong-Gyu and Cho, Myeong-Cheoul
Journal: Gene (2014): 494-9
Deep sequencing analysis of mutations resulting from the incorporation of dNTP analogs.
Authors: Petrie, Katherine L and Joyce, Gerald F
Journal: Nucleic acids research (2010): 8095-104
Design and synthesis of a photocleavable fluorescent nucleotide 3'-O-allyl-dGTP-PC-Bodipy-FL-510 as a reversible terminator for DNA sequencing by synthesis.
Authors: Meng, Qinglin and Kim, Dae Hyun and Bai, Xiaopeng and Bi, Lanrong and Turro, Nicholas J and Ju, Jingyue
Journal: The Journal of organic chemistry (2006): 3248-52