7-Hydroxy-4-methylcoumarin [4-Methylumbelliferone] Fluorescence reference standard

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Email	sales@aatbio.com
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Physical properties

Molecular weight	176.17
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.1
Extinction coefficient (cm ^-1 M ^-1)	18000
Excitation (nm)	360
Emission (nm)	448

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

90-33-5

Molecular weight

176.17

Correction Factor (280 nm)

0.1

Extinction coefficient (cm ^-1 M ^-1)

18000

Excitation (nm)

360

Emission (nm)

448

This compound is used as a calibration standard for 7-hydroxy-4-methylcoumarin-based enzyme substrates. It has pH-dependent fluorescence.

Platform

Fluorescence microplate reader

Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxy-4-methylcoumarin [4-Methylumbelliferone] *Fluorescence reference standard* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	567.634 µL	2.838 mL	5.676 mL	28.382 mL	56.763 mL
5 mM	113.527 µL	567.634 µL	1.135 mL	5.676 mL	11.353 mL
10 mM	56.763 µL	283.817 µL	567.634 µL	2.838 mL	5.676 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (280 nm)	0.1
Extinction coefficient (cm ^-1 M ^-1)	18000
Excitation (nm)	360
Emission (nm)	448

Images

Figure 1. Chemical structure for 7-Hydroxy-4-methylcoumarin [4-Methylumbelliferone] *Fluorescence reference standard*

References

View all 19 references: Citation Explorer

Excited state properties of 7-hydroxy-4-methylcoumarin in the gas phase and in solution. A theoretical study
Authors: Georgieva I, Trendafilova N, Aquino A, Lischka H.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860

Novel thiocoumarins as inhibitors of TNF-alpha induced ICAM-1 expression on human umbilical vein endothelial cells (HUVECs) and microsomal lipid peroxidation
Authors: Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.
Journal: Bioorg Med Chem (2005): 1605

Stability of the complexes of some lanthanides with coumarin derivatives. I. Cerium(III)-4-methyl-7-hydroxycoumarin
Authors: Kostova IP, Manolov, II, Radulova MK.
Journal: Acta Pharm (2004): 37

Measurement of enzyme kinetics using microscale steady-state kinetic analysis
Authors: Gleason NJ, Carbeck JD.
Journal: Langmuir (2004): 6374

Structural requirements of hydroxylated coumarins for in vitro anti-Helicobacter pylori activity
Authors: Kawase M, Tanaka T, Sohara Y, Tani S, Sakagami H, Hauer H, Chatterjee SS.
Journal: In Vivo (2003): 509

Crystal structure of 7,8-dihydroxy-4-methylcoumarin
Authors: Kurosaki H, Sharma RK, Otsuka M, Goto M.
Journal: Anal Sci (2003): 647

Modifying rates of reductive elimination of leaving groups from indolequinone prodrugs: a key factor in controlling hypoxia-selective drug release
Authors: Everett SA, Swann E, Naylor MA, Stratford MR, Patel KB, Tian N, Newman RG, Vojnovic B, Moody CJ, Wardman P.
Journal: Biochem Pharmacol (2002): 1629

Effects of 18 alpha-glycyrrhizic acid on rat liver cytochrome P450 isoenzymes and phase II transferase
Authors: Yang J, Peng RX, Kong R, Yu JP.
Journal: Yao Xue Xue Bao (2001): 321

Synthesis and evaluation of novel fluorogenic substrates for the detection of bacterial beta-galactosidase
Authors: Chilvers KF, Perry JD, James AL, Reed RH.
Journal: J Appl Microbiol (2001): 1118

Antioxidative and prooxidative effects of coumarin derivatives on free radical initiated and photosensitized peroxidation of human low-density lipoprotein
Authors: Liu ZQ, Yu W, Liu ZL.
Journal: Chem Phys Lipids (1999): 125