Name: 7-Hydroxy-4-methylcoumarin [4-Methylumbelliferone] *Fluorescence reference standard*
Brand: AAT Bioquest
SKU: 41
Price: 90 USD
Availability: InStock

7-Hydroxy-4-methylcoumarin

4-Methylumbelliferone; Fluorescence reference standard

This compound is used as a calibration standard for 7-hydroxy-4-methylcoumarin-based enzyme substrates. It has pH-dependent fluorescence.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
41	1 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxy-4-methylcoumarin [4-Methylumbelliferone] *Fluorescence reference standard* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	567.634 µL	2.838 mL	5.676 mL	28.382 mL	56.763 mL
5 mM	113.527 µL	567.634 µL	1.135 mL	5.676 mL	11.353 mL
10 mM	56.763 µL	283.817 µL	567.634 µL	2.838 mL	5.676 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	176.17 g/mol	=	mL	x	mM	=	mmol

References

View all 19 references: Citation Explorer

Excited state properties of 7-hydroxy-4-methylcoumarin in the gas phase and in solution. A theoretical study

Authors:

Georgieva I, Trendafilova N, Aquino A, Lischka H.

Journal:

J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860

Novel thiocoumarins as inhibitors of TNF-alpha induced ICAM-1 expression on human umbilical vein endothelial cells (HUVECs) and microsomal lipid peroxidation

Authors:

Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.

Journal:

Bioorg Med Chem (2005): 1605

Stability of the complexes of some lanthanides with coumarin derivatives. I. Cerium(III)-4-methyl-7-hydroxycoumarin

Authors:

Kostova IP, Manolov, II, Radulova MK.

Journal:

Acta Pharm (2004): 37

Measurement of enzyme kinetics using microscale steady-state kinetic analysis

Authors:

Gleason NJ, Carbeck JD.

Journal:

Langmuir (2004): 6374

Structural requirements of hydroxylated coumarins for in vitro anti-Helicobacter pylori activity

Authors:

Kawase M, Tanaka T, Sohara Y, Tani S, Sakagami H, Hauer H, Chatterjee SS.

Journal:

In Vivo (2003): 509

Physical properties

Molecular weight	176.17
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.1
Extinction coefficient (cm ^-1 M ^-1)	18000
Excitation (nm)	360
Emission (nm)	448

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	90-33-5

Instrument settings

Fluorescence microplate reader
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

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Page updated on October 8, 2025