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7-Hydroxy-4-methylcoumarin [4-Methylumbelliferone] *Fluorescence reference standard*
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Application notes
This compound is used as a calibration standard for 7-hydroxy-4-methylcoumarin-based enzyme substrates. It has pH-dependent fluorescence.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxy-4-methylcoumarin [4-Methylumbelliferone] *Fluorescence reference standard* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 567.634 µL | 2.838 mL | 5.676 mL | 28.382 mL | 56.763 mL |
| 5 mM | 113.527 µL | 567.634 µL | 1.135 mL | 5.676 mL | 11.353 mL |
| 10 mM | 56.763 µL | 283.817 µL | 567.634 µL | 2.838 mL | 5.676 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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References
View all 19 references: Citation Explorer
Excited state properties of 7-hydroxy-4-methylcoumarin in the gas phase and in solution. A theoretical study
Authors: Georgieva I, Trendafilova N, Aquino A, Lischka H.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860
Authors: Georgieva I, Trendafilova N, Aquino A, Lischka H.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860
Novel thiocoumarins as inhibitors of TNF-alpha induced ICAM-1 expression on human umbilical vein endothelial cells (HUVECs) and microsomal lipid peroxidation
Authors: Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.
Journal: Bioorg Med Chem (2005): 1605
Authors: Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.
Journal: Bioorg Med Chem (2005): 1605
Stability of the complexes of some lanthanides with coumarin derivatives. I. Cerium(III)-4-methyl-7-hydroxycoumarin
Authors: Kostova IP, Manolov, II, Radulova MK.
Journal: Acta Pharm (2004): 37
Authors: Kostova IP, Manolov, II, Radulova MK.
Journal: Acta Pharm (2004): 37
Measurement of enzyme kinetics using microscale steady-state kinetic analysis
Authors: Gleason NJ, Carbeck JD.
Journal: Langmuir (2004): 6374
Authors: Gleason NJ, Carbeck JD.
Journal: Langmuir (2004): 6374
Structural requirements of hydroxylated coumarins for in vitro anti-Helicobacter pylori activity
Authors: Kawase M, Tanaka T, Sohara Y, Tani S, Sakagami H, Hauer H, Chatterjee SS.
Journal: In Vivo (2003): 509
Authors: Kawase M, Tanaka T, Sohara Y, Tani S, Sakagami H, Hauer H, Chatterjee SS.
Journal: In Vivo (2003): 509