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7-Hydroxy-4-methylcoumarin [4-Methylumbelliferone] *Fluorescence reference standard*

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Physical properties
Molecular weight176.17
Spectral properties
Correction Factor (280 nm)0.1
Extinction coefficient (cm -1 M -1)18000
Excitation (nm)360
Emission (nm)448
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Coumarins
Molecular weight
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
This compound is used as a calibration standard for 7-hydroxy-4-methylcoumarin-based enzyme substrates. It has pH-dependent fluorescence.


Fluorescence microplate reader

Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxy-4-methylcoumarin [4-Methylumbelliferone] *Fluorescence reference standard* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM567.634 µL2.838 mL5.676 mL28.382 mL56.763 mL
5 mM113.527 µL567.634 µL1.135 mL5.676 mL11.353 mL
10 mM56.763 µL283.817 µL567.634 µL2.838 mL5.676 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles


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Spectral properties

Correction Factor (280 nm)0.1
Extinction coefficient (cm -1 M -1)18000
Excitation (nm)360
Emission (nm)448



View all 19 references: Citation Explorer
Excited state properties of 7-hydroxy-4-methylcoumarin in the gas phase and in solution. A theoretical study
Authors: Georgieva I, Trendafilova N, Aquino A, Lischka H.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860
Novel thiocoumarins as inhibitors of TNF-alpha induced ICAM-1 expression on human umbilical vein endothelial cells (HUVECs) and microsomal lipid peroxidation
Authors: Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.
Journal: Bioorg Med Chem (2005): 1605
Stability of the complexes of some lanthanides with coumarin derivatives. I. Cerium(III)-4-methyl-7-hydroxycoumarin
Authors: Kostova IP, Manolov, II, Radulova MK.
Journal: Acta Pharm (2004): 37
Measurement of enzyme kinetics using microscale steady-state kinetic analysis
Authors: Gleason NJ, Carbeck JD.
Journal: Langmuir (2004): 6374
Structural requirements of hydroxylated coumarins for in vitro anti-Helicobacter pylori activity
Authors: Kawase M, Tanaka T, Sohara Y, Tani S, Sakagami H, Hauer H, Chatterjee SS.
Journal: In Vivo (2003): 509
Crystal structure of 7,8-dihydroxy-4-methylcoumarin
Authors: Kurosaki H, Sharma RK, Otsuka M, Goto M.
Journal: Anal Sci (2003): 647
Modifying rates of reductive elimination of leaving groups from indolequinone prodrugs: a key factor in controlling hypoxia-selective drug release
Authors: Everett SA, Swann E, Naylor MA, Stratford MR, Patel KB, Tian N, Newman RG, Vojnovic B, Moody CJ, Wardman P.
Journal: Biochem Pharmacol (2002): 1629
Effects of 18 alpha-glycyrrhizic acid on rat liver cytochrome P450 isoenzymes and phase II transferase
Authors: Yang J, Peng RX, Kong R, Yu JP.
Journal: Yao Xue Xue Bao (2001): 321
Synthesis and evaluation of novel fluorogenic substrates for the detection of bacterial beta-galactosidase
Authors: Chilvers KF, Perry JD, James AL, Reed RH.
Journal: J Appl Microbiol (2001): 1118
Antioxidative and prooxidative effects of coumarin derivatives on free radical initiated and photosensitized peroxidation of human low-density lipoprotein
Authors: Liu ZQ, Yu W, Liu ZL.
Journal: Chem Phys Lipids (1999): 125