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7-Hydroxycoumarin-4-acetic acid, succinimidyl ester

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Physical properties
Molecular weight317.25
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
7-Hydroxycoumarin-4-acetic acid is used for labeling peptides and proteins to give blue fluorescence. It can also be used for labeling other biological molecules.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxycoumarin-4-acetic acid, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM315.209 µL1.576 mL3.152 mL15.76 mL31.521 mL
5 mM63.042 µL315.209 µL630.418 µL3.152 mL6.304 mL
10 mM31.521 µL157.604 µL315.209 µL1.576 mL3.152 mL

Molarity calculator

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View all 5 references: Citation Explorer
Stopped-flow kinetic analysis of long-chain fatty acid dissociation from bovine serum albumin
Authors: Demant EJ, Richieri GV, Kleinfeld AM.
Journal: Biochem J (2002): 809
Continuous recording of long-chain acyl-coenzyme a synthetase activity using fluorescently labeled bovine serum albumin
Authors: Demant EJ, Nystrom BT.
Journal: Anal Biochem (2001): 38
Bovine serum albumin-(7-hydroxycoumarin-4-acetic acid) complex: applications to the fluorometric measurement of fatty acid concentrations
Authors: Demant EJ., undefined
Journal: Anal Biochem (1999): 366
Covalent complexes between serum albumin and 7-hydroxycoumarin-4-acetic acid: synthesis and applications in the spectrophotometric detection of long-chain fatty acids
Authors: Demant EJ., undefined
Journal: Biochim Biophys Acta (1996): 43
Structure-activity relationship of coumarins in xanthine oxidase inhibition
Authors: Chang WS, Chiang HC.
Journal: Anticancer Res (1995): 1969