7-Hydroxycoumarin-4-acetic acid, succinimidyl ester

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Physical properties

Molecular weight	317.25
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

317.25

7-Hydroxycoumarin-4-acetic acid is used for labeling peptides and proteins to give blue fluorescence. It can also be used for labeling other biological molecules.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxycoumarin-4-acetic acid, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	315.209 µL	1.576 mL	3.152 mL	15.76 mL	31.521 mL
5 mM	63.042 µL	315.209 µL	630.418 µL	3.152 mL	6.304 mL
10 mM	31.521 µL	157.604 µL	315.209 µL	1.576 mL	3.152 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Images

Figure 1. Chemical structure for 7-Hydroxycoumarin-4-acetic acid, succinimidyl ester

References

View all 5 references: Citation Explorer

Stopped-flow kinetic analysis of long-chain fatty acid dissociation from bovine serum albumin
Authors: Demant EJ, Richieri GV, Kleinfeld AM.
Journal: Biochem J (2002): 809

Continuous recording of long-chain acyl-coenzyme a synthetase activity using fluorescently labeled bovine serum albumin
Authors: Demant EJ, Nystrom BT.
Journal: Anal Biochem (2001): 38

Bovine serum albumin-(7-hydroxycoumarin-4-acetic acid) complex: applications to the fluorometric measurement of fatty acid concentrations
Authors: Demant EJ., undefined
Journal: Anal Biochem (1999): 366

Covalent complexes between serum albumin and 7-hydroxycoumarin-4-acetic acid: synthesis and applications in the spectrophotometric detection of long-chain fatty acids
Authors: Demant EJ., undefined
Journal: Biochim Biophys Acta (1996): 43

Structure-activity relationship of coumarins in xanthine oxidase inhibition
Authors: Chang WS, Chiang HC.
Journal: Anticancer Res (1995): 1969