Name: ReadiCleave™ iFluor 546 AML-NHS ester
Brand: AAT Bioquest
SKU: 7006
Price: 430 USD
Availability: InStock

ReadiCleave™ iFluor 546 AML-NHS ester

Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis or another detection mode. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. ReadiCleave™ iFluor® 546 AML contains an azidomethyl linker that can be cleaved with TCEP to remove the iFluor® 546 fluorophore from the target molecule. The cleavage can be carried out by adding 10-100 mM TCEP solution (pH 7.5) and incubating at 65 °C for 1-5 min. iFluor® 546 is a superior replacement to Alexa Fluor® 546. iFluor® 546 and Alexa Fluor® 546 have very similar spectral properties.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
7006	1 mg	Price

References

View all 40 references: Citation Explorer

Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.

Authors:

Ahishali, Bulent and Kaya, Mehmet

Journal:

Methods in molecular biology (Clifton, N.J.) (2021): 87-103

Effects of Viscosity and Refractive Index on the Emission and Diffusion Properties of Alexa Fluor 405 Using Fluorescence Correlation and Lifetime Spectroscopies.

Authors:

van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G

Journal:

Journal of fluorescence (2021): 835-845

Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.

Authors:

Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben

Journal:

Chemphyschem : a European journal of chemical physics and physical chemistry (2021)

Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568.

Authors:

Gajdos, Tamás and Hopp, Béla and Erdélyi, Miklós

Journal:

Journal of fluorescence (2020): 437-443

Photo-isomerization of the Cyanine Dye Alexa-Fluor 647 (AF-647) in the Context of dSTORM Super-Resolution Microscopy.

Authors:

Karlsson, Joshua K G and Laude, Alex and Hall, Michael J and Harriman, Anthony

Journal:

Chemistry (Weinheim an der Bergstrasse, Germany) (2019): 14983-14998

Physical properties

Molecular weight	1494.76
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.25
Correction factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	541
Emission (nm)	557
Quantum yield	0.67 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025