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Dansyl-X, SE

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Physical properties
Molecular weight461.53
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.387
Extinction coefficient (cm -1 M -1)3300
Excitation (nm)335
Emission (nm)518
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
461.53
Correction Factor (280 nm)
0.387
Extinction coefficient (cm -1 M -1)
3300
Excitation (nm)
335
Emission (nm)
518
Dansyl-X SE has higher labeling efficiency and better stability than Dansyl chloride. Dansyl dyes have environmentally sensitive fluorescence quantum yields and emission maxima along with large Stokes shifts. This environment-sensitive fluorescence property has made Dansyl dyes an important tool for biophysical studies. They are particularly useful for preparing fluorescent drug or ligand analogs that are expected to bind to hydrophobic sites in proteins or membranes or other biological receptors. Dansyl protein conjugates have fluorescence lifetimes of 10-20 nanoseconds.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Dansyl-X, SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM216.671 µL1.083 mL2.167 mL10.834 mL21.667 mL
5 mM43.334 µL216.671 µL433.341 µL2.167 mL4.333 mL
10 mM21.667 µL108.335 µL216.671 µL1.083 mL2.167 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.387
Extinction coefficient (cm -1 M -1)3300
Excitation (nm)335
Emission (nm)518

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
Dansyl-X, acid33551833000.387

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References


View all 33 references: Citation Explorer
Phenylisothiocyanate and dansyl chloride precolumn derivatizations for the high-performance liquid chromatography analysis of the antitumoral agent ES-285 in dog plasma
Authors: Maraschiello C, Mir and a E, Millan E, Floriano P, Vilageliu J.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 1
Dansyl chloride derivatization of methamphetamine: a method with advantages for screening and analysis of methamphetamine in urine
Authors: Yamada H, Yamahara A, Yasuda S, Abe M, Oguri K, Fukushima S, Ikeda-Wada S.
Journal: J Anal Toxicol (2002): 17
Determination of the xenoestrogens 4-nonylphenol and bisphenol A by high-performance liquid chromatography and fluorescence detection after derivatisation with dansyl chloride
Authors: Naassner M, Mergler M, Wolf K, Schuphan I.
Journal: J Chromatogr A (2002): 133
Automated pre-column derivatization of amines in biological samples with dansyl chloride and with or without post-column chemiluminescence formation by using TCPO-H2O2
Authors: Molins-Legua C, Campins-Falco P, Sevillano-Cabeza A.
Journal: Analyst (1998): 2871
A new screening method for amphetamine and methamphetamine using dansyl chloride derivatization and cartridge fluorescence
Authors: Yamada H, Ikeda-Wada S, Oguri K.
Journal: Biol Pharm Bull (1998): 778
Determination of L-buthionin (SR)-sulfoximine, gamma-glutamylcysteine synthetase inhibitor in rat plasma with HPLC after prelabeling with dansyl chloride
Authors: Koyama H, Sugioka N, Hirata I, Ohta T, Kishimoto H.
Journal: J Chromatogr Sci (1996): 326
Dansyl chloride labelling of stratum corneum: its rapid extraction from skin can predict skin irritation due to surfactants and cleansing products
Authors: Paye M, Simion FA, Pierard GE.
Journal: Contact Dermatitis (1994): 91
Dihydroxyacetone test as a substitute for the dansyl chloride test
Authors: Pierard GE, Pierard-Franchimont C.
Journal: Dermatology (1993): 133
Microscopic evaluation of the dansyl chloride test
Authors: Pierard GE., undefined
Journal: Dermatology (1992): 37
Determination of the insecticide promecarb by fluorogenic labelling with dansyl chloride
Authors: Garcia Sanchez F, Cruces Blanco C.
Journal: Analyst (1991): 851