Aminopropargyl ddATP [7-Deaza-7-Propargylamino-2',3'-dideoxyadenosine-5'-triphosphate]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 615.19 |
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Dideoxynucleotides (ddNTPs), Nucleic Acid Building Blocks, RNA Purification & Analysis, Sanger Sequencing
CAS 114748-69-5 | Molecular weight 615.19 |
Sanger method is one of the most reliable and earliest DNA sequencing methods. DNA is synthesized from four deoxynucleotide triphosphates (dNTPs). Each new nucleotide is added to the 3′ -OH group of the last dNTP added. Dideoxythymidine triphosphates (ddTTPs) can be added to the growing DNA strand but when it is, chain elongation stops because there is no 3′ -OH for the next nucleotide to be attached to. The DNA to be sequenced is prepared as a single strand. This template DNA is supplied with a mixture of dATP, dGTP, dCTP and dTTP in ample quantities. A mixture of all four dideoxynucleotides (ddATP, ddGTP, ddCTP and ddTTP), each present in limiting quantities and each labeled with a "tag" that fluoresces a different color, are added. Because all four normal nucleotides are present, chain elongation proceeds normally until, by chance, DNA polymerase inserts a ddNTP (instead of the normal dNTPs). If the ratio of normal nucleotide to the dideoxy versions is high enough, some DNA strands will succeed in adding several hundred nucleotides before insertion of the ddNTPs halts the process. At the end of the incubation period, the fragments are separated by length from longest to shortest. The resolution is so good that a difference of one nucleotide is enough to separate that strand from the next shorter and next longer strand. Each of the four ddNTPs fluoresces a different color when illuminated by a laser beam and an automatic scanner provides a printout of the sequence. These ddATP, ddGTP, ddCTP and ddTTP amine derivatives are the essential building blocks for developing Sanger sequencing reagents.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Aminopropargyl ddATP [7-Deaza-7-Propargylamino-2',3'-dideoxyadenosine-5'-triphosphate] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 162.551 µL | 812.757 µL | 1.626 mL | 8.128 mL | 16.255 mL |
5 mM | 32.51 µL | 162.551 µL | 325.103 µL | 1.626 mL | 3.251 mL |
10 mM | 16.255 µL | 81.276 µL | 162.551 µL | 812.757 µL | 1.626 mL |
Molarity calculator
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Images
Citations
View all 27 citations: Citation Explorer
Polyadenylated sequencing primers enable complete readability of PCR amplicons analyzed by dideoxynucleotide sequencing
Authors: Beranek, M., Drastikova, M., Petera, J.
Journal: Acta Medica (Hradec Kralove) (2012): 160-4
Authors: Beranek, M., Drastikova, M., Petera, J.
Journal: Acta Medica (Hradec Kralove) (2012): 160-4
UV-induced bond modifications in thymine and thymine dideoxynucleotide: structural elucidation of isomers by differential mobility mass spectrometry
Authors: St-Jacques, A., Anichina, J., Schneider, B. B., Covey, T. R., Bohme, D. K.
Journal: Anal Chem (2010): 6163-7
Authors: St-Jacques, A., Anichina, J., Schneider, B. B., Covey, T. R., Bohme, D. K.
Journal: Anal Chem (2010): 6163-7
Analysis of processivity of mungbean dideoxynucleotide-sensitive DNA polymerase and detection of the activity and expression of the enzyme in the meristematic and meiotic tissues and following DNA damaging agent
Authors: Roy, S., Choudhury, S. R., Sengupta, D. N.
Journal: Arch Biochem Biophys (2008): 55-65
Authors: Roy, S., Choudhury, S. R., Sengupta, D. N.
Journal: Arch Biochem Biophys (2008): 55-65
A dideoxynucleotide-sensitive DNA polymerase activity characterized from endoreduplicating cells of mungbean (Vigna radiata L.) during ontogeny of cotyledons
Authors: Roy, S., Sarkar, S. N., Singh, S. K., Sengupta, D. N.
Journal: FEBS J (2007): 2005-23
Authors: Roy, S., Sarkar, S. N., Singh, S. K., Sengupta, D. N.
Journal: FEBS J (2007): 2005-23
Mechanism-based suppression of dideoxynucleotide resistance by K65R human immunodeficiency virus reverse transcriptase using an alpha-boranophosphate nucleoside analogue
Authors: Selmi, B., Boretto, J., Sarfati, S. R., Guerreiro, C., Canard, B.
Journal: J Biol Chem (2001): 48466-72
Authors: Selmi, B., Boretto, J., Sarfati, S. R., Guerreiro, C., Canard, B.
Journal: J Biol Chem (2001): 48466-72
Synthesis of the first ferrocene-labeled dideoxynucleotide and its use for 3'-redox end-labeling of 5'-modified single-stranded oligonucleotides
Authors: Anne, A., Blanc, B., Moiroux, J.
Journal: Bioconjug Chem (2001): 396-405
Authors: Anne, A., Blanc, B., Moiroux, J.
Journal: Bioconjug Chem (2001): 396-405
Improving dideoxynucleotide-triphosphate utilisation by the hyper-thermophilic DNA polymerase from the archaeon Pyrococcus furiosus
Authors: Evans, S. J., Fogg, M. J., Mamone, A., Davis, M., Pearl, L. H., Connolly, B. A.
Journal: Nucleic Acids Res (2000): 1059-66
Authors: Evans, S. J., Fogg, M. J., Mamone, A., Davis, M., Pearl, L. H., Connolly, B. A.
Journal: Nucleic Acids Res (2000): 1059-66
Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation
Authors: Li, Y., Mitaxov, V., Waksman, G.
Journal: Proc Natl Acad Sci U S A (1999): 9491-6
Authors: Li, Y., Mitaxov, V., Waksman, G.
Journal: Proc Natl Acad Sci U S A (1999): 9491-6
Characterization of the native and recombinant catalytic subunit of human DNA polymerase gamma: identification of residues critical for exonuclease activity and dideoxynucleotide sensitivity
Authors: Longley, M. J., Ropp, P. A., Lim, S. E., Copel and , W. C.
Journal: Biochemistry (1998): 10529-39
Authors: Longley, M. J., Ropp, P. A., Lim, S. E., Copel and , W. C.
Journal: Biochemistry (1998): 10529-39
Comparative performance of high-density oligonucleotide sequencing and dideoxynucleotide sequencing of HIV type 1 pol from clinical samples
Authors: Gunthard, H. F., Wong, J. K., Ignacio, C. C., Havlir, D. V., Richman, D. D.
Journal: AIDS Res Hum Retroviruses (1998): 869-76
Authors: Gunthard, H. F., Wong, J. K., Ignacio, C. C., Havlir, D. V., Richman, D. D.
Journal: AIDS Res Hum Retroviruses (1998): 869-76
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
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