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Aminopropargyl dGTP [5-Propargylamino-2'-deoxyguanosine-5'-triphosphate]

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Physical properties
Molecular weight582.99
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
The amine-modified deoxyguanosine 5'-triphosphates (such as 5-propargylamino-2'-deoxyguanosine-5'-triphosphate) can be used to produce amine-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. Aminopropargyl dGTP can be readily incorporated into DNA through the conventional enzymatic incorporation techniques. The resulting amine-modified nucleic acids can then be labeled using any of amine-reactive fluorescent dyes, biotins and other amine-reactive reagents. The aminopropargyl-modified nucleotides can be incorporated to extremely high and consistent levels compared to the tag-labeled dGTP that generally has higher stereo-hindrance. Subsequent reaction of the amine-modified nucleic acid with an excess of amine-reactive reagent achieves correspondingly high and consistent labeling efficiencies, regardless of the labeling reagent chosen. This two-step labeling method also eliminates the need to optimize an enzymatic reaction to accommodate different dye-modified nucleotides, which may incorporate at very different rates. This labeling method is widely used for both FISH probes and microarray-based experiments.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Aminopropargyl dGTP [5-Propargylamino-2'-deoxyguanosine-5'-triphosphate] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM171.53 µL857.648 µL1.715 mL8.576 mL17.153 mL
5 mM34.306 µL171.53 µL343.059 µL1.715 mL3.431 mL
10 mM17.153 µL85.765 µL171.53 µL857.648 µL1.715 mL

Molarity calculator

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View all 3 references: Citation Explorer
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Fluorescent labelling of cRNA for microarray applications
Authors: t Hoen PA, de Kort F, van Ommen GJ, den Dunnen JT.
Journal: Nucleic Acids Res (2003): e20
Sequence-specific scission of DNA by the chemical nuclease activity of 1,10-phenanthroline-copper(I) targeted by RNA
Authors: Chen CB, Gorin MB, Sigman DS.
Journal: Proc Natl Acad Sci U S A (1993): 4206