AMP-Fluorescein conjugate calibrator

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Chemical structure for AMP-Fluorescein conjugate calibrator
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Unit Size: Cat No: Price (USD): Qty:
100 nmol 13610 $295


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Overview

Ex/Em (nm)492/515
MW~800
SolventDMSO
Storage Freeze (<-15 °C)
Minimize light exposure
Category Enzyme Detection
Phosphodiesterases
Related Secondary Reagents
AMP-Fluorescein conjugate calibrator is desigend to use with our green fluorescent FAM-cAMP PDE IV substrate (#Cat: 13602), a cAMP derivative that is a specific substrate for phosphodiesterase (PDE) IV. This AMP-Fluorescein conjugate calibrator is the hydrolyzed product of FAM-cAMP PDE IV substrate by PDE IV. It is used to calibrate the hydrolysis rate of FAM-cAMP PDE IV substrate by PDE IV.




Calculators
Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AMP-Fluorescein conjugate calibrator to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =
 






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Wavelength (nm)





References & Citations

cAMP is a ligand for the tandem GAF domain of human phosphodiesterase 10 and cGMP for the tandem GAF domain of phosphodiesterase 11
Authors: Gross-Langenhoff M, Hofbauer K, Weber J, Schultz A, Schultz JE.
Journal: J Biol Chem (2006): 2841

cAMP phosphodiesterase activity evaluation in human carcinoma of salivary glands
Authors: Spoto G, della Malva M, Rubini C, Fioroni M, Piattelli A, Serra E, Di Nicola M, Santoleri F.
Journal: Nucleosides Nucleotides Nucleic Acids (2006): 1113

cAMP phosphodiesterase-4A1 (PDE4A1) has provided the paradigm for the intracellular targeting of phosphodiesterases, a process that underpins compartmentalized cAMP signalling
Authors: Huston E, Houslay TM, Baillie GS, Houslay MD.
Journal: Biochem Soc Trans (2006): 504

Characterization of a novel cAMP-binding, cAMP-specific cyclic nucleotide phosphodiesterase (TcrPDEB1) from Trypanosoma cruzi
Authors: Diaz-Benjumea R, Laxman S, Hinds TR, Beavo JA, Rascon A.
Journal: Biochem J (2006): 305

Compartmentalization of cAMP-Dependent Signaling by Phosphodiesterase-4D Is Involved in the Regulation of Vasopressin-Mediated Water Reabsorption in Renal Principal Cells
Authors: Stefan E, Wiesner B, Baillie GS, Mollajew R, Henn V, Lorenz D, Furkert J, Santamaria K, Nedvetsky P, Hundsrucker C, Beyermann M, Krause E, Pohl P, Gall I, Macintyre AN, Bachmann S, Houslay MD, Rosenthal W, Klussmann E.
Journal: J Am Soc Nephrol. (2006)

DdPDE4, a novel cAMP-specific phosphodiesterase at the surface of dictyostelium cells
Authors: Bader S, Kortholt A, Snippe H, Van Haastert PJ.
Journal: J Biol Chem (2006): 20018

Expression and Activity of cAMP Phosphodiesterase Isoforms in Pulmonary Artery Smooth Muscle Cells from Patients with Pulmonary Hypertension: Role for PDE1
Authors: Murray F, Patel HH, Suda RY, Zhang S, Thistlethwaite P, Yuan JX, Insel PA.
Journal: Am J Physiol Lung Cell Mol Physiol. (2006)

Expression of cAMP and cGMP-phosphodiesterase isoenzymes 3, 4, and 5 in the human clitoris: immunohistochemical and molecular biology study
Authors: Oelke M, Hedlund P, Albrecht K, Ellinghaus P, Stief CG, Jonas U, Andersson KE, Uckert S.
Journal: Urology (2006): 1111

Expression of cAMP and cGMP-phosphodiesterase isoenzymes 3, 4, and 5 in the human clitoris: immunohistochemical and molecular biology study
Authors: Sampaio F.
Journal: Int Braz J Urol (2006): 483

Helix-1 of the cAMP-specific phosphodiesterase PDE4A1 regulates its phospholipase-D-dependent redistribution in response to release of Ca2+
Authors: Huston E, Gall I, Houslay TM, Houslay MD.
Journal: J Cell Sci (2006): 3799


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Additional Documents

 
Safety Data Sheet (SDS)


Catalogs
1. Enzyme Probes & Assay Kits

Certificate of Analysis