Name: Amplite® Colorimetric D-Lactate Assay Kit
Brand: AAT Bioquest
SKU: 13811
Price: 355 USD
Availability: InStock

Amplite® Colorimetric D-Lactate Assay Kit

Amplite® Colorimetric D-Lactate Assay Kit is a colorimetric assay kit for detecting D-lactate in biological and cell culture samples using an enzyme-coupled NADH readout.

Colorimetric detection: Measures D-lactate through a chromogenic NADH sensor in an enzyme-coupled reaction.
High sensitivity: Detects D-lactate concentrations as low as 4 µM in a 100 µL reaction volume.
D-lactate specific: Designed for selective measurement of the D-lactate isomer.
Ratiometric readout: Measure absorbance at 575/605 nm using a clear-bottom plate.
Applications: Suitable for D-lactate quantitation in serum, plasma, urine, and cell culture samples.

D-lactate dose response was measured with Amplite® Colorimetric D-lactate Assay Kit in a 96-well white clear bottom plate using a SpectraMax Plus (Molecular Devices) microplate reader.

Protocol

SDS

Catalog	Size	Price	Quantity
13811	200 Tests	Price

Overview

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid. Lactate is constantly produced from pyruvate via the enzyme lactate dehydrogenase (LDH) in the process of metabolism and exercise. Monitoring lactate levels is a good way to evaluate the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is not metabolized by mammals and its elimination from the body depends mainly on renal excretion. D- and L-lactic acid are found in many fermented milk products such as yogurt and cheese, and also in pickled vegetables, and cured meats and fish. The D- and L-lactic acid (generated by bacteria) is a quality indicator of foods, such as egg, milk, fruit juice and wine. Abnormal high concentration of D-lactate in the blood is usually a reflection of bacterial overgrowth in the gastrointestinal tract. AAT Bioquest's Amplite® Lactate Assay Kits (Cat# 13814 and 13815 for L-lactate assay, and Cat# 13810 and 13811 for D-lactate assay) provide both fluorescence and absorbance-based method for detecting either L-lactate or D-lactate in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, lactate is proportionally related to NADH, which is specifically monitored by a chromogenic NADH sensor. The signal can easily read by an absorbance microplate reader at the absorbance ratio of ~A575nm/A605nm to increase assay sensitivity. With this Colorimetric Amplite® D-Lactate Assay Kit, we were able to detect as little as 4 µM D-lactate in a 100 µL reaction volume.

Citations

View all 27 citations: Citation Explorer

ROS-driven mitochondrial and mtDNA release as a novel pathway of metabolic injury in diabetes

Authors:

Chen, Dandan and Jia, Yuxin and Duan, Xiaohong and Huang, Jianhua and Deng, Xiaohong

Journal:

Journal of Molecular Endocrinology (2026): JME--25

Guiqi Baizhu prescription attenuates 5-FU-induced intestinal mucositis by targeting IKK$\beta$ to inhibit M1 macrophage polarization

Authors:

Zhou, Yu-Cen and Li, Ya-Ling and Ma, Jing and Li, Junjie and Si, Qi and Wang, Shunzhi and Huang, Dan and Wa, Xiao-Xia and Cai, Hui and Liu, Yong-Qi

Journal:

Chinese Medicine (2026): 194

A centrifugal bioreactor for immunotherapy: modeling and optimization of the growth of human T lymphoblastic leukemia cells

Authors:

Fraser-Hevlin, Brenden and Kaiphanliam, Kitana M and Moore, Zakora and Davis, William C and Van Wie, Bernard J

Journal:

Biotechnology and Bioprocess Engineering (2026): 1--16

Zinc-loaded montmorillonite alleviates avian pathogenic Escherichia coli-induced intestinal barrier damage in broiler chickens

Authors:

Zha, Pingping and Liu, Xinghuo and Qin, Zihao and Xue, Yu and Tan, Zichao and Chen, Yueping and Zhou, Yanmin

Journal:

Journal of Animal Science (2025): skaf327

Evaluation of a Burkholderia ambifaria strain from plants as a novel promising probiotic in dental caries management

Authors:

Chen, Zirang and Lu, Yangyu and Xu, Zhezhen and Wu, Lijing and Wei, Xi and Cai, Yanling

Journal:

Journal of Oral Microbiology (2024): 2420612

References

View all 72 references: Citation Explorer

DNA binding, antioxidant, cytotoxicity (MTT, lactate dehydrogenase, NO), and cellular uptake studies of structurally different nickel(II) thiosemicarbazone complexes: synthesis, spectroscopy, electrochemistry, and X-ray crystallography

Authors:

Prabhakaran R, Kalaivani P, Huang R, Poornima P, Vijaya Padma V, Dallemer F, Natarajan K.

Journal:

J Biol Inorg Chem (2013): 233

Serum lactate dehydrogenase predicts for overall survival benefit in patients with metastatic renal cell carcinoma treated with inhibition of mammalian target of rapamycin

Authors:

Armstrong AJ, George DJ, Halabi S.

Journal:

J Clin Oncol (2012): 3402

Production of monoclonal antibodies for Plasmodium vivax lactate dehydrogenase and patient sera screening using sandwich ELISA

Authors:

Kim JH, Lee J, Sohn HJ, Song HO, Kim JY, Lee WJ, Park H, Shin HJ.

Journal:

Parasitol Res (2012): 1645

Adult-onset multiple acyl CoA dehydrogenation deficiency associated with an abnormal isoenzyme pattern of serum lactate dehydrogenase

Authors:

Sugai F, Baba K, Toyooka K, Liang WC, Nishino I, Yamadera M, Sumi H, Fujimura H, Nishikawa Y.

Journal:

Neuromuscul Disord (2012): 159

Towards improved prognostic scores predicting survival in patients with brain metastases: a pilot study of serum lactate dehydrogenase levels

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Nieder C, Marienhagen K, Dalhaug A, Norum J.

Journal:

ScientificWorldJournal (2012): 609323

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	575/605 nm
Recommended plate	Clear bottom

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Email	sales@aatbio.com
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Page updated on October 7, 2026

Component A: Enzyme Mix	2 bottles (lyophilized powder)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: NAD	1 vial
Component D: D-Lactate Standard	2.25 mg/vial