Name: Amplite® Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit
Brand: AAT Bioquest
SKU: 13809
Price: 355 USD
Availability: InStock

Amplite® Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit

Amplite® Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit is a colorimetric assay kit for detecting D-LDH activity in biological and cell culture samples through an enzyme-coupled NADH readout.

Colorimetric detection: Monitors NADH with a chromogenic sensor to quantify D-LDH activity.
High sensitivity: Detects D-LDH activity as low as 3 mU/mL in a 100 µL reaction volume.
D-LDH specific: Designed for selective measurement of D-lactate dehydrogenase activity.
Ratiometric readout: Measure absorbance at 575/605 nm using a clear-bottom plate.
Applications: Suitable for D-LDH measurements in serum, plasma, urine, and cell culture samples.

D-LDH dose response was measured with Amplite® Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit in a 96-well white wall/clear bottom plate using a SpectraMax Plus (Molecular Devices) microplate reader.

Protocol

SDS

Catalog	Size	Price	Quantity
13809	200 Tests	Price

Overview

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyzes the interconversion of pyruvate and lactate. LDH is present in cytosol of a wide variety of organisms, including animals and plants. Cells release LDH into the bloodstream after tissue damage or red blood cell hemolysis. Since LDH is a fairly stable enzyme, it has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has a broad range of applications. LDH is also elevated in certain pathological conditions such as cancer. This Amplite® Lactate Dehydrogenase Assay Kit provides an absorption-based method for detecting D-lactate dehydrogenase (D-LDH) in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, LDH is proportionally related to the concentration of NADH that is specifically monitored by a chromogenic NADH sensor. This assay is specific for D-LDH. The absorption signal can be read by an absorption microplate reader at the absorbance ratio of ~570 nm to ~605 nm (A575 nm/A605 nm). With this colorimetric Amplite® D-lactate Dehydrogenase Assay Kit, we were able to detect as little as 3 mU/mL D-lactate dehydrogenase in a 100 µL reaction volume.

Citations

View all 10 citations: Citation Explorer

Characterization of antihypertensive and cardioprotective effects of extra virgin olive oil against doxorubicin induced cardiomyopathy in rats

Authors:

AlMalki, Waleed Hassan and Shahid, Imran

Journal:

Journal of Pharmacy \& Pharmacognosy Research (2020): 316--326

Arenobufagin inhibits prostate cancer epithelial-mesenchymal transition and metastasis by down-regulating $\beta$-catenin

Authors:

Chen, Liping and Mai, Weiqian and Chen, Minfeng and Hu, Jianyang and Zhuo, Zhenjian and Lei, Xueping and Deng, Lijuan and Liu, Junshan and Yao, Nan and Huang, Maohua and others,

Journal:

Pharmacological Research (2017): 130--142

Arenobufagin inhibits prostate cancer epithelial-mesenchymal transition and metastasis by down-regulating β-catenin

Authors:

Chen, Liping and Mai, Weiqian and Chen, Minfeng and Hu, Jianyang and Zhuo, Zhenjian and Lei, Xueping and Deng, Lijuan and Liu, Junshan and Yao, Nan and Huang, Maohua and others, undefined

Journal:

Pharmacological Research (2017)

Fibroblast Activation Protein Alpha-Activated Tripeptide Bufadienolide Anti-tumor Prodrug with Reduced Cardiotoxicity

Authors:

Deng, Li-Juan and Wang, Long-Hai and Peng, Cheng-Kang and Li, Yi-Bin and Huang, Mao-Hua and Chen, Min-Feng and Lei, Xue-Ping and Qi, Ming and Cen, Yun and Ye, Wen-Cai and others, undefined

Journal:

Journal of Medicinal Chemistry (2017)

The use of KnockOut serum replacement (KSR) in three dimentional rat testicular cells co-culture model: An improved male reproductive toxicity testing system

Authors:

Zhang, Xiaofang and Wang, Lei and Zhang, Xiaodong and Ren, Lijun and Shi, Wenjing and Tian, Yijun and Zhu, Jiangbo and Zhang, Tianbao

Journal:

Food and Chemical Toxicology (2017)

References

View all 72 references: Citation Explorer

DNA binding, antioxidant, cytotoxicity (MTT, lactate dehydrogenase, NO), and cellular uptake studies of structurally different nickel(II) thiosemicarbazone complexes: synthesis, spectroscopy, electrochemistry, and X-ray crystallography

Authors:

Prabhakaran R, Kalaivani P, Huang R, Poornima P, Vijaya Padma V, Dallemer F, Natarajan K.

Journal:

J Biol Inorg Chem (2013): 233

Serum lactate dehydrogenase predicts for overall survival benefit in patients with metastatic renal cell carcinoma treated with inhibition of mammalian target of rapamycin

Authors:

Armstrong AJ, George DJ, Halabi S.

Journal:

J Clin Oncol (2012): 3402

Production of monoclonal antibodies for Plasmodium vivax lactate dehydrogenase and patient sera screening using sandwich ELISA

Authors:

Kim JH, Lee J, Sohn HJ, Song HO, Kim JY, Lee WJ, Park H, Shin HJ.

Journal:

Parasitol Res (2012): 1645

Adult-onset multiple acyl CoA dehydrogenation deficiency associated with an abnormal isoenzyme pattern of serum lactate dehydrogenase

Authors:

Sugai F, Baba K, Toyooka K, Liang WC, Nishino I, Yamadera M, Sumi H, Fujimura H, Nishikawa Y.

Journal:

Neuromuscul Disord (2012): 159

Towards improved prognostic scores predicting survival in patients with brain metastases: a pilot study of serum lactate dehydrogenase levels

Authors:

Nieder C, Marienhagen K, Dalhaug A, Norum J.

Journal:

ScientificWorldJournal (2012): 609323

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	575/605 nm
Recommended plate	Clear bottom

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Page updated on October 4, 2026

Component A: Enzyme Probe	1 bottle (lyophilized powder)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: NAD	1 vial
Component D: D-lactate Dehydrogenase	10 U/vial