Amplite® Colorimetric Endotoxin Detection Kit

Colorimetric detection: Endotoxin Yellow™ generates an intense yellow product in the presence of endotoxin and LAL.
High sensitivity: Detects E. coli endotoxin as low as 0.001 EU/mL after 10 minutes.
LAL-based chemistry: Endotoxin-dependent substrate hydrolysis produces a signal proportional to endotoxin activity.
Microplate compatible: Read absorbance at 498 nm using a clear-bottom plate.
Applications: Suitable for endotoxin testing in protein, peptide, antibody, and other biological samples.

E.coli Endotoxin dose response was measured in an all-clear 96-well plate using a with a Spectrum Max microplate reader (Molecular Devices) at OD 498nm. As low as 0.001 EU/mL of E.coli Endotoxin can be detected with 10 minutes incubation (n=3).

Protocol

SDS

COO

COA

Catalog	Size	Price	Quantity
60007	100 Tests	Price

Overview

Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membranes of Gram-negative bacteria. LPS is a potent stimulator of the vertebrate innate immune system and can cause fever, septic shock and eventually death. It is also recognized as a biomarker for the detection of bacterial pathogen invasion, and responsible for the development of inflammatory response and endotoxic shock in extreme cases. Detection of LPS in biological materials, such as protein, peptide or antibody sample, is a critical task in biomanufacturing and bioprocessing. Amplite®™ Colorimetric Endotoxin Detection Kit uses Endotoxin Yellow™, a sensitive chromogenic substrate. Endotoxin Yellow™ can be hydrolyzed in the presence of endotoxins and the Limulus Amebocyte Lysate (LAL), an extract of blood cells from a horseshoe crab, to generate an intense yellow colored product. The endotoxin activity is proportional to the absorbance of the yellow product resulted from the hydrolysis of Endotoxin Yellow™. Amplite®™ Colorimetric Endotoxin Detection Kit can detect a broad range of endotoxin from 1 EU/ml to 0.002 EU/ml.

Citations

View all 35 citations: Citation Explorer

Exosomal Tenascin-C Primes Macrophage Pyroptosis Amplifying Aberrant Inflammation during Sepsis-Induced Acute Lung Injury

Authors:

Gong, Ting and Zhang, Xuedi and Liu, Xiaolei and Ye, Yinfeng and Tian, Zhiyuan and Yin, Shuang and Zhang, Min and Tang, Jing and Liu, Youtan

Journal:

Translational Research (2024)

Macrophage-derived exosomal aminopeptidase N aggravates sepsis-induced acute lung injury by regulating necroptosis of lung epithelial cell

Authors:

Gong, Ting and Zhang, Xuedi and Peng, Zhiyong and Ye, Yinfeng and Liu, Ruimeng and Yang, Yinggui and Chen, Zhugui and Zhang, Zhihao and Hu, Hongfei and Yin, Shuang and others,

Journal:

Communications Biology (2022): 1--17

A Validation Study of the Limulus Amebocyte Lysate Test as an End-Product Endotoxin Test for Polyvalent Horse Snake Antivenom

Authors:

Sheraba, N. S., Diab, M. R., Yassin, A. S., Amin, M. A., Zedan, H. H.

Journal:

PDA J Pharm Sci Technol (2019): 562-571

Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples

Authors:

Uchida, T., Kaku, Y., Hayasaka, H., Kofuji, M., Momose, N., Miyazawa, H., Ueda, Y., Ito, K., Ookawara, S., Morishita, Y.

Journal:

Med Devices (Auckl) (2019): 429-433

Evaluation of a portable test system for assessing endotoxin activity in raw milk

Authors:

Suzuki, Y., Suzuki, K., Shimamori, T., Tsuchiya, M., Niehaus, A., Lakritz, J.

Journal:

J Vet Med Sci (2016): 49-53

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	498 nm
Recommended plate	Clear bottom

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Page updated on October 10, 2026

Component A: Endotoxin Yellow™	1 Vial
Component B: Endotoxin-Free Water	1 Bottle (25 mL)
Component C: Limulus Amebocyte Lysate	1 Vial
Component D: E.coli Endotoxin Standard	1 Vial (100 EU/mL, 100 µL)
Component E: DMSO	1 Vial