Amplite® Colorimetric Hydrogen Peroxide Assay Kit

Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. It is involved in a number of biological events that have been linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down's syndrome. Perhaps the most intriguing aspect of H2O2 biology is the recent report that antibodies have the capacity to convert molecular oxygen into hydrogen peroxide to contribute to the normal recognition and destruction processes of the immune system. Measurement of this reactive species will help to determine how oxidative stress modulates varied intracellular pathways. This Amplite® Colorimetric Hydrogen Peroxide Assay Kit uses our unique Amplite® IR peroxidase substrate to quantify hydrogen peroxide in solutions and cell extracts. Upon hydrogen peroxide oxidation the colorless Amplite® IR generates an intense blue color product that is pH-independent from pH 4 to 10. The existing colorimetric hydrogen peroxide assays (from other vendors) often have severe sample interferences caused by the inherent absorption of biological samples. The near infrared absorption of Amplite® IR product minimizes the assay background that since the biological samples rarely absorb light beyond 600 nm. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized 'mix and read' assay that is compatible with HTS liquid handling instruments.

Hydrogen Peroxide dose response was measured in a a white wall/clear bottom 96-well plate with the Amplite® Colorimetric Hydrogen Peroxide Assay Kit using a Spectramax absorbance microplate reader (Molecular Devices).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11500	500 Tests	Price

Citations

View all 6 citations: Citation Explorer

Anti-inflammatory effects of cold atmospheric plasma irradiation on the THP-1 human acute monocytic leukemia cell line

Authors:

Hirasawa, Ito and Odagiri, Haruka and Park, Giri and Sanghavi, Rutvi and Oshita, Takaya and Togi, Akiko and Yoshikawa, Katsunori and Mizutani, Koji and Takeuchi, Yasuo and Kobayashi, Hiroaki and others,

Journal:

PLOS ONE (2023): e0292267

Macrophage metabolism reprogramming EGCG-Cu coordination capsules delivered in polyzwitterionic hydrogel for burn wound healing and regeneration

Authors:

Li, Qinghua and Song, Huijuan and Li, Shuangyang and Hu, Pengbo and Zhang, Chuangnian and Zhang, Ju and Feng, Zujian and Kong, Deling and Wang, Weiwei and Huang, Pingsheng

Journal:

Bioactive Materials (2023): 251--264

One-dimensional array of nanoparticles using segmented polyurethane nanofibers as a template for enhanced catalytic efficiency

Authors:

Meboso, Taichi and Goto, Satoshi and Takamura, Eiichiro and Sakamoto, Hiroaki

Journal:

Colloid and Polymer Science (2023): 1--7

The oxidoreductase CLIC4 is required to maintain mitochondrial function and resistance to exogenous oxidants in breast cancer cells

Authors:

Al Khamici, Heba and Sanchez, Vanesa C and Yan, Hualong and Cataisson, Christophe and Michalowski, Aleksandra M and Yang, Howard H and Li, Luowei and Lee, Maxwell P and Huang, Jing and Yuspa, Stuart H

Journal:

Journal of Biological Chemistry (2022)

Mild Oxidative Stress Reduces NRF2 SUMOylation to Promote Kras/Lkb1/Keap1 Mutant Lung Adenocarcinoma Cell Migration and Invasion

Authors:

Xu, Jiaqian and Guo, Haoyan and Xing, Zhengcao and Zhang, Wenlong and He, Jianli and Cheng, Jinke and Cai, Rong

Journal:

Oxidative medicine and cellular longevity (2020)

References

View all 152 references: Citation Explorer

Comparative effects of alpha-tocopherol and gamma-tocotrienol against hydrogen peroxide induced apoptosis on primary-cultured astrocytes

Authors:

Mazlan M, Sue Mian T, Mat Top G, Zurinah Wan Ngah W.

Journal:

J Neurol Sci (2006): 5

Effect of antisense oligonucleotide against Smac/DIABLO on inhibition of hydrogen peroxide induced myocardial apoptosis of neonatal rats

Authors:

Liang PF, Huang XY, Long JH, Xiao MZ, Yang XH, Zhang PH.

Journal:

Zhonghua Shao Shang Za Zhi (2006): 175

Cardioprotective role of endogenous hydrogen peroxide during ischemia-reperfusion injury in canine coronary microcirculation in vivo

Authors:

Yada T, Shimokawa H, Hiramatsu O, Haruna Y, Morita Y, Kashihara N, Shinozaki Y, Mori H, Goto M, Ogasawara Y, Kajiya F.

Journal:

Am J Physiol Heart Circ Physiol (2006): H1138

Simple and rapid determination of hydrogen peroxide using phosphine-based fluorescent reagents with sodium tungstate dihydrate

Authors:

Onoda M, Uchiyama T, Mawatari K, Kaneko K, Nakagomi K.

Journal:

Anal Sci (2006): 815

Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes

Authors:

Bienert GP, Moller AL, Kristiansen KA, Schulz A, Moller IM, Schjoerring JK, Jahn TP.

Journal:

J Biol Chem. (2006)

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Absorbance microplate reader
Absorbance	650 nm
Recommended plate	Clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025

Component A: Amplite™ IR Peroxidase Substrate	1 vial
Component B: H2O2	1 vial (3% stabilized solution, 200 µL)
Component C: Assay Buffer	1 bottle (100 mL)
Component D: Horseradish Peroxidase	1 vial (20 units)
Component E: DMSO	1 vial (0.5 mL)