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Amplite® Colorimetric L-Alanine Assay Kit
L-alanine (L-Ala) plays a crucial role as a building block of important proteins. L-alanine is mostly synthesized by the muscle cells from lactic acid and absorbed into blood via the liver. It is converted into pyruvate by glutamic-pyruvic transaminase to enter the metabolic mainstream. L-Ala is critical for the production of glucose and hence blood sugar management, and plays an important role on the immune system and prevention of kidney stones. Insufficiency of L-alanine is usually a sign of poor nutrition, low protein diet, as well as stress. AAT Bioquest's Amplite® Colorimetric L-Alanine Assay Kit offers a sensitive colorimetric assay for quantifying L-alanine in biological samples. It utilizes an enzyme coupled reaction that releases hydrogen peroxide, which can be detected by Quest Fluor™ L-Alanine Sensor in an absorbance microplate reader at 575 nm.
L-alanine dose response was measured at 30-minute intervals using Amplite® Colorimetric L-Alanine Assay Kit on a white clear 96-well plate using a SpectraMax microplate reader (Molecular Devices).
L-alanine dose response was measured at 30-minute intervals using Amplite® Colorimetric L-Alanine Assay Kit on a white clear 96-well plate using a SpectraMax microplate reader (Molecular Devices).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
13826200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Absorbance microplate reader
Absorbance575 nm
Recommended plateClear bottom
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Page updated on October 5, 2025