Name: Amplite® Colorimetric Tyrosinase Assay Kit
Brand: AAT Bioquest
SKU: 11311
Price: 465 USD
Availability: InStock

Amplite® Colorimetric Tyrosinase Assay Kit

Amplite® Colorimetric Tyrosinase Assay Kit provides a fast, sensitive method to monitor tyrosinase activity in a range of research and industrial contexts.

High sensitivity: Detects low levels of tyrosinase activity with strong signal-to-background ratio
One-step protocol: Simple mix-and-read format minimizes hands-on time and streamlines workflows
Application versatility: Ideal for melanoma research, tyrosinase inhibitor screening, and pigment pathway analysis
Comparable alternative: Offers a reliable solution similar to Abcam's tyrosinase activity assay kits

Tyrosinase dose response was measured with Amplite® Colorimetric Tyrosinase Assay Kit in a 96-well white plate using a SpectraMax microplate reader (Molecular Devices). Equal volume of Tyrosinase standards and Tyrosinase Substrate with enhancer were added and incubated for 30 minutes at room temperature. The signal was acquired at 510 nm.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11311	100 Tests	Price

Overview

Amplite® Colorimetric Tyrosinase Assay Kit is a simple, one-step assay optimized for the reliable detection of tyrosinase activity in biological samples. Tyrosinase plays a critical role in melanin biosynthesis and is a key target in drug discovery, cancer research, and cosmetic science. This assay utilizes a proprietary colorless substrate that, upon enzymatic reaction with tyrosinase, generates a chromogenic product with strong absorbance at 510 nm. The increase in absorbance is directly proportional to tyrosinase activity, allowing sensitive quantification using a standard microplate reader. The kit is ideal for screening tyrosinase inhibitors, studying melanoma-related illnesses, and assessing enzyme activity in pigment-related pathways across pharmaceutical and food industries.

Citations

View all 10 citations: Citation Explorer

A comprehensive review on tyrosinase inhibitors

Authors:

Zolghadri, S., Bahrami, A., Hassan Khan, M. T., Munoz-Munoz, J., Garcia-Molina, F., Garcia-Canovas, F., Saboury, A. A.

Journal:

J Enzyme Inhib Med Chem (2019): 279-309

Tyrosinase (TYR) gene sequencing and literature review reveals recurrent mutations and multiple population founder gene mutations as causative of oculocutaneous albinism (OCA) in Pakistani families

Authors:

Shakil, M., Harlalka, G. V., Ali, S., Lin, S., D'Atri, I., Hussain, S., Nasir, A., Shahzad, M. A., Ullah, M. I., Self, J. E., Baple, E. L., Crosby, A. H., Mahmood, S.

Journal:

Eye (Lond) (2019): ersion="1.0" encoding="UTF-8" ?>11311.enlEndN

Moraceae Plants with Tyrosinase Inhibitory Activity: A Review

Authors:

Burl, undefined and o, B., Clericuzio, M., Cornara, L.

Journal:

Mini Rev Med Chem (2017): 108-121

Tyrosinase inhibitors: a patent review (2011-2015)

Authors:

Ullah, S., Son, S., Yun, H. Y., Kim, D. H., Chun, P., Moon, H. R.

Journal:

Expert Opin Ther Pat (2016): 347-62

Critical review of Ayurvedic Varnya herbs and their tyrosinase inhibition effect

Authors:

Sharma, K., Joshi, N., Goyal, C.

Journal:

Anc Sci Life (2015): 18-25

Storage, safety and handling

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	510 nm
Recommended plate	White plate/Clear bottom

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Page updated on October 13, 2025

Component A: Tyrosinase Standard	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Tyrosinase Substrate	1 vial
Component D: Tyrosinase Enhancer	1 vial (100 µL)