Amplite® Colorimetric Tyrosinase Assay Kit

Amplite® Colorimetric Tyrosinase Assay Kit provides a fast, sensitive method to monitor tyrosinase activity in a range of research and industrial contexts.

High sensitivity: Detects low levels of tyrosinase activity with strong signal-to-background ratio
One-step protocol: Simple mix-and-read format minimizes hands-on time and streamlines workflows
Application versatility: Ideal for melanoma research, tyrosinase inhibitor screening, and pigment pathway analysis
Comparable alternative: Offers a reliable solution similar to Abcam's tyrosinase activity assay kits

Product description

Amplite® Colorimetric Tyrosinase Assay Kit is a simple, one-step assay optimized for the reliable detection of tyrosinase activity in biological samples. Tyrosinase plays a critical role in melanin biosynthesis and is a key target in drug discovery, cancer research, and cosmetic science. This assay utilizes a proprietary colorless substrate that, upon enzymatic reaction with tyrosinase, generates a chromogenic product with strong absorbance at 510 nm.

The increase in absorbance is directly proportional to tyrosinase activity, allowing sensitive quantification using a standard microplate reader. The kit is ideal for screening tyrosinase inhibitors, studying melanoma-related illnesses, and assessing enzyme activity in pigment-related pathways across pharmaceutical and food industries.

Example protocol

AT A GLANCE

Protocol Summary

Prepare and add standards and samples (50 µL)
Prepare and add Tyrosinase Substrate working solution to the standards and samples wells (50 µL)
Incubate the plate at room temperature for 30 to 60 minutes
Monitor the absorbance at 510 nm

Important Bring all the kit components at room temperature before starting the experiment.

PREPARATION OF STOCK SOLUTIONS

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

1. Tyrosinase stock solution (2000 U/mL)

Add 120 µL Assay Buffer (Component B) into Tyrosinase Standard (Component A) and mix well.
Note Store the unused Tyrosinase stock solution at -20 °C in single use aliquots.

2. Tyrosinase Substrate stock solution (50X)

Add 100 µL ddH₂O into Assay Substrate (Component C) and mix well.
Note Store the unused Tyrosinase Substrate stock solution at -20 °C in single use aliquots.

PREPARATION OF STANDARD SOLUTION

For convenience, use the Serial Dilution Planner:
https://www.aatbio.com/tools/serial-dilution/11311

Tyrosinase standard

Use Tyrosinase stock solution (2000 U/mL) and Assay Buffer to generate 400 U/mL final concentration of Tyrosinase Standard solution (T1). Then perform 1:2 serial dilutions to get remaining serially diluted Tyrosinase Standards (T2-T7). Note: The final in well concentration of the standards will be 2X. Note: With provided standard, 2 standard curves can be performed in duplicates if using at suggested concentrations.

PREPARATION OF WORKING SOLUTION

Tyrosinase Substrate working solution

Make a 1:50 dilution by adding 20 µL Tyrosinase Substrate stock solution (50X) and 20 µL Tyrosinase Enhancer (Component D) into 1 mL Assay Buffer (Component B) and mix well.
Note Tyrosinase Substrate working solution should be made immediately upon use. We recommend using working solution within several hours.

SAMPLE EXPERIMENTAL PROTOCOL

Table 1.Layout of Tyrosinase standards and test samples in a white-clear bottom 96- wells microplate. Tyrosinase standards (T1-T7= 400 to 6.25 U/mL), TS= Test Samples, BL= Blank samples

T1	T1	TS	TS
T2	T2
T3	T3
T4	T4
T5	T5
T6	T6
T7	T7
BL	BL

The following protocol can be used as a guideline and should be optimized according to the needs.

Prepare the standards and test samples as per recommendations in assay buffer and add 50 µL of each in a microplate.
Add 50 µL Tyrosinase Substrate working solution to the wells of standards and samples.
Incubate the reaction at room temperature for 30 to 60 minutes.
Monitor the absorbance with an absorbance plate reader at 510 nm.

Citations

View all 10 citations: Citation Explorer

A comprehensive review on tyrosinase inhibitors
Authors: Zolghadri, S., Bahrami, A., Hassan Khan, M. T., Munoz-Munoz, J., Garcia-Molina, F., Garcia-Canovas, F., Saboury, A. A.
Journal: J Enzyme Inhib Med Chem (2019): 279-309

Tyrosinase (TYR) gene sequencing and literature review reveals recurrent mutations and multiple population founder gene mutations as causative of oculocutaneous albinism (OCA) in Pakistani families
Authors: Shakil, M., Harlalka, G. V., Ali, S., Lin, S., D'Atri, I., Hussain, S., Nasir, A., Shahzad, M. A., Ullah, M. I., Self, J. E., Baple, E. L., Crosby, A. H., Mahmood, S.
Journal: Eye (Lond) (2019): ersion="1.0" encoding="UTF-8" ?>11311.enlEndN

Moraceae Plants with Tyrosinase Inhibitory Activity: A Review
Authors: Burl, undefined and o, B., Clericuzio, M., Cornara, L.
Journal: Mini Rev Med Chem (2017): 108-121

Tyrosinase inhibitors: a patent review (2011-2015)
Authors: Ullah, S., Son, S., Yun, H. Y., Kim, D. H., Chun, P., Moon, H. R.
Journal: Expert Opin Ther Pat (2016): 347-62

Critical review of Ayurvedic Varnya herbs and their tyrosinase inhibition effect
Authors: Sharma, K., Joshi, N., Goyal, C.
Journal: Anc Sci Life (2015): 18-25

Page updated on August 13, 2025

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