Name: Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit
Brand: AAT Bioquest
SKU: 13804
Price: 348 USD
Availability: InStock

Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells. G6P may also be converted to glycogen or starch for storage in the liver and muscles. G6P is utilized by glucose-6-phosphate dehydrogenase (G6PD) to generate the reducing equivalents in the form of NADPH. This is particularly important in red blood cells where G6PD deficiency leads to hemolytic anemia. AAT Bioquest's Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit provides a simple, sensitive and rapid fluorescence-based method for detecting G6P in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the coupled enzyme assay, the G6P concentration is proportionally related to NADPH that is specifically monitored by a fluorogenic NADPH sensor. The fluorescence signal can be read by a fluorescence microplate reader. With the Amplite® G6P Assay Kit, we were able to detect as little as 0.3 µM G6P in a 100 µL reaction volume.

G6P dose response was measured with Amplite® Fluorimetric G6P Assay Kit in a 96-well solid black plate using a Gemini (Molecular Devices) microplate reader.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13804	200 Tests	Price

References

View all 84 references: Citation Explorer

The microsomal enzyme 17beta-hydroxysteroid dehydrogenase 3 faces the cytoplasm and uses NADPH generated by glucose-6-phosphate dehydrogenase

Authors:

Legeza B, Balazs Z, Nashev LG, Odermatt A.

Journal:

Endocrinology (2013): 205

Molecular characterization of glucose-6-phosphate dehydrogenase deficient variants in Baghdad city - Iraq

Authors:

Al-Musawi BM, Al-Allawi N, Abdul-Majeed BA, Eissa AA, Jubrael JM, Hamamy H.

Journal:

BMC Blood Disord (2012): 4

High prevalence of hemoglobin disorders and glucose-6-phosphate dehydrogenase (G6PD) deficiency in the Republic of Guinea (West Africa)

Authors:

Millimono TS, Loua KM, Rath SL, Relvas L, Bento C, Diakite M, Jarvis M, Daries N, Ribeiro LM, Manco L, Kaeda JS.

Journal:

Hemoglobin (2012): 25

Effects of laparoscopic Roux-en-Y gastric bypass on glucose-6 phosphate dehydrogenase activity in obese type 2 diabetics

Authors:

Schneider AM, Rawat D, Weinstein LS, Gupte SA, Richards WO.

Journal:

Surg Endosc (2012): 823

A novel cytofluorometric assay for the detection and quantification of glucose-6-phosphate dehydrogenase deficiency

Authors:

Shah SS, Diakite SA, Traore K, Diakite M, Kwiatkowski DP, Rockett KA, Wellems TE, Fairhurst RM.

Journal:

Sci Rep (2012): 299

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Absorbance microplate reader
Absorbance	575/605 nm
Recommended plate	Clear bottom

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 13, 2025

Component A: Enzyme Probe	2 bottles (lyophilized powder)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: NADP	1 vial
Component D: Glucose-6-Phosphate Standard	3.04 mg/vial