Amplite® Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*

Amplite® Fluorimetric Peroxynitrite Quantification Kit

Green Fluorescence

Amplite® Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence* is a fluorimetric assay kit for quantification of peroxynitrite (ONOO⁻) in solution.

Detection Principle: DAX-J2™ PON Green 99 reacts with ONOO⁻ to generate a bright green fluorescent product.
Specificity: Selectively detects ONOO⁻ over other reactive oxygen and nitrogen species.
Detection Chemistry: Uses DAX-J2™ PON Green 99 as the peroxynitrite-responsive fluorescent probe.
Instrument Compatibility: Fluorescence detection at Ex/Em 490/530 nm using a microplate reader or spectrometer.

Peroxynitrite was measured with Amplite® Fluorimetric Peroxynitrite Quantification Kit on a solid black 96-well plate using a Gemini microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
16316	100 Tests	Price

Overview

Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent. Peroxynitrite is formed from the reaction between superoxide radicals and nitric oxide generated in cells. It can damage a wide array of biomolecules including proteins, enzymes, lipids and nucleic acids, eventually contributing to cell death. Due to its extremely short half-life and low steady-state concentration, it has been challenging to detect and quantify peroxynitrite in solution. In order to address this need, AAT Bioquest's Amplite® Fluorimetric Peroxynitrite Quantification Kit provides a sensitive tool to measure ONOO- in solution. DAX-J2™ PON Green 99 reacts with ONOO- to generate a bright green fluorescent product. It specifically reacts with ONOO- with high selectivity over other reactive oxygen species (ROS) and reactive nitrogen species (RNS). This kit can be used with a fluorescence microplate reader and spectrometer.

Citations

View all 8 citations: Citation Explorer

Triterpenoids and ultrasound dual-catalytic nanoreactor ignites long-lived hypertoxic reactive species storm for deep tumor treatment

Authors:

Li, Ziying and Xie, Huanzhang and Shi, Huifang and Li, Dongmiao and Zhang, Zizhong and Chen, Haijun and Gao, Yu

Journal:

Chemical Engineering Journal (2023): 139938

Mechanisms of oxidative removal of 1, 4-dioxane via free chlorine rapidly mixing into monochloramine: Implications on water treatment and reuse

Authors:

Wu, Liang and Patton, Samuel D and Liu, Haizhou

Journal:

Journal of Hazardous Materials (2022): 129760

Interactions between pH, reactive species, and cells in plasma-activated water can remove algae

Authors:

Mizoi, Ken and Rodr{\'\i}guez-Gonz{\'a}lez, Vicente and Sasaki, Mao and Suzuki, Shoki and Honda, Kaede and Ishida, Naoya and Suzuki, Norihiro and Kuchitsu, Kazuyuki and Kondo, Takeshi and Yuasa, Makoto and others,

Journal:

RSC advances (2022): 7626--7634

Nanoparticles Encapsulating Nitrosylated Maytansine To Enhance Radiation Therapy

Authors:

Gao, Shi and Zhang, Weizhong and Wang, Renjie and Hopkins, Sean P and Spagnoli, Jonathan C and Racin, Mohammed and Bai, Lin and Li, Lu and Jiang, Wen and Yang, Xueyuan and others,

Journal:

ACS nano (2020): 1468--1481

Peroxynitrite Generation and Increased Heterotrophic Capacity Are Linked to the Disruption of the Coral--Dinoflagellate Symbiosis in a Scleractinian and Hydrocoral Species

Authors:

Marangoni, Laura Fern and es de Barros , undefined and Mies, Miguel and Güth, Arthur Z and Banha, Thomás NS and Inague, Alex and Fonseca, Juliana da Silva and Dalmolin, Camila and Faria, Samuel Coelho and Ferrier-Pagès, Christine and Bianchini, Adalto

Journal:

Microorganisms (2019): 426

References

View all 10 references: Citation Explorer

Imaging of nucleolar RNA in living cells using a highly photostable deep-red fluorescent probe

Authors:

Zhou B, Liu W, Zhang H, Wu J, Liu S, Xu H, Wang P.

Journal:

Biosens Bioelectron (2015): 189

RNA and DNA binding of inert oligonuclear ruthenium(II) complexes in live eukaryotic cells

Authors:

Li X, Gorle AK, Ainsworth TD, Heimann K, Woodward CE, Collins JG, Keene FR.

Journal:

Dalton Trans (2015): 3594

Low molecular weight fluorescent probes with good photostability for imaging RNA-rich nucleolus and RNA in cytoplasm in living cells

Authors:

Song G, Sun Y, Liu Y, Wang X, Chen M, Miao F, Zhang W, Yu X, Jin J.

Journal:

Biomaterials (2014): 2103

Luminescence of [Ru(bpy)2(dppz)]2+ bound to RNA mismatches

Authors:

McConnell AJ, Song H, Barton JK.

Journal:

Inorg Chem (2013): 10131

Co-aggregation of RNA binding proteins in ALS spinal motor neurons: evidence of a common pathogenic mechanism

Authors:

Keller BA, Volkening K, Droppelmann CA, Ang LC, Rademakers R, Strong MJ.

Journal:

Acta Neuropathol (2012): 733

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	530 nm
Cutoff	515 nm
Recommended plate	Solid black

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Fax
Email	sales@aatbio.com
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Custom size	Inquire
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 9, 2026

Component A: DAX-J2™ PON Green 99	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: DMSO	1 vial (100 µL)