Amplite® Fluorimetric Pyruvate Assay Kit

Amplite® Fluorimetric Pyruvate Assay Kit is a fluorimetric assay kit for quantification of pyruvate in biological samples.

Detection Principle: Uses an enzyme-coupled reaction that generates hydrogen peroxide for fluorescent pyruvate detection.
Detection Chemistry: Uses Quest Fluor™ Pyruvate Sensor with two enzyme mixes.
Plate Format: Compatible with 96- and 384-well microplates.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm using solid black microplates.

Pyruvate dose response was measured with the Amplite® Fluorimetric Pyruvate Assay Kit on a solid black 96-well plate using a Gemini microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13820	200 Tests	Price

Overview

Pyruvate is an important chemical compound in intracellular metabolic pathways. It is derived from metabolism of glucose known as glycolysis. One molecule of glucose breaks down into two molecules of pyruvate, which supplies living cells energy through one of two ways. When oxygen is present (aerobic respiration), pyruvate is converted into acetyl-CoA by pyruvate dehydrogenase which enters citric acid cycles (also known as the Krebs cycle) to generate ATP. When there is insufficient oxygen is available, the pyruvate is broken down anaerobically, creating lactate in animals and ethanol in plants and microorganisms. Abnormal levels of pyruvate, or concentration ratio of lactate-to-pyruvate may be linked to liver disease or metabolic disorders and it is a diagnostic measurement in patient's clinical and other laboratory studies. AAT Bioquest's Amplite® Fluorimetric Pyruvate Assay Kit offers a sensitive fluorimetric assay for quantifying pyruvate in the samples. It utilizes an enzyme coupled reaction that releases hydrogen peroxide, which can be detected by pyruvate sensor by an absorbance microplate reader at 575 nm.

Citations

View all 2 citations: Citation Explorer

Inhibition of the Monocarboxylate Transporter 1 (MCT1) Promotes 3T3-L1 Adipocyte Proliferation and Enhances Insulin Sensitivity

Authors:

Bailey, Tracey and Nieto, Ainhoa and McDonald, Patricia

Journal:

International Journal of Molecular Sciences (2022): 1901

Interference of reversible redox compounds in enzyme catalysed assays--Electrochemical limitations

Authors:

McBeth, Craig and Stott-Marshall, Robert J

Journal:

Analytical Biochemistry (2022): 114972

References

View all 51 references: Citation Explorer

A replaceable dual-enzyme capillary microreactor using magnetic beads and its application for simultaneous detection of acetaldehyde and pyruvate

Authors:

Shi J, Zhao W, Chen Y, Guo L, Yang L.

Journal:

Electrophoresis (2012): 2145

Detection of inflammatory arthritis by using hyperpolarized 13C-pyruvate with MR imaging and spectroscopy

Authors:

MacKenzie JD, Yen YF, Mayer D, Tropp JS, Hurd RE, Spielman DM.

Journal:

Radiology (2011): 414

Amplex UltraRed enhances the sensitivity of fluorimetric pyruvate detection

Authors:

Zhu A, Romero R, Petty HR.

Journal:

Anal Biochem (2010): 123

Comparison of high-performance liquid chromatography with fluorescence detection versus enzymatic assay to measure blood pyruvate in clinical practice

Authors:

Wolff F, El Khattabi C, Bourdon F, Willems D.

Journal:

Clin Biochem (2009): 1099

Faecal tumour pyruvate kinase M2: not a good marker for the detection of colorectal adenomas

Authors:

Shastri YM, Stein JM.

Journal:

Br J Cancer (2008): 1366; author reply 1367

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 13, 2026

Component A: Quest Fluor™ Pyruvate Sensor	1 vial
Component B1: Enzyme Mix 1	2 bottles (lyophilized powder)
Component B2: Enzyme Mix 2	2 vials (lyophilized powder)
Component C: Assay Buffer	1 bottle (10 mL)
Component D: Pyruvate Standard	1 vial (100 mM, 100 µL)
Component E: DMSO	1 vial (100 µL)