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Amplite® Universal Fluorimetric Protease Activity Assay Kit
Red Fluorescence
Monitoring of various protease activities has become a routine task for many biological laboratories. Our Amplite® Universal Fluorimetric Protease Activity Assay Kits are an ideal choice for performing routine assays necessary during the isolation of proteases, or for identifying the presence of contaminating proteases in protein samples. The kits use fluorescent casein conjugates that are proven to be a generic substrate for a broad spectrum of proteases. In the intact substrate, casein is heavily labeled with a fluorescent dye, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity. The kits provide all the essential components with an optimized "mix & read" protocol that can be easily automated to HTS instruments.
Trypsin protease activity was analyzed by using Amplite® Universal Fluorimetric Protease Activity Assay Kit. Protease substrate was incubated with 3 units of trypsin. The control wells had protease substrate only (without trypsin). The fluorescence signal was measured starting from time 0 when trypsin was added. Samples were done in triplicate.
Trypsin protease activity was analyzed by using Amplite® Universal Fluorimetric Protease Activity Assay Kit. Protease substrate was incubated with 3 units of trypsin. The control wells had protease substrate only (without trypsin). The fluorescence signal was measured starting from time 0 when trypsin was added. Samples were done in triplicate.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
13501500 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.32
Correction factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)
90000
Excitation (nm)552
Emission (nm)578
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on October 13, 2025