Amplite® West ECL HRP Substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability. It is used for detection of peroxidase activity from HRP-conjugated antibodies and probes on nitrocellulose or PVDF membranes. The substrate provides a quick and efficient way for detecting proteins expressed at femtogram levels. It does not require any expensive instruments and consumables and is compatible with the existing instruments commonly used for western blots. When combined with very diluted and optimized antibody concentrations and blocking buffers, Amplite® West ECL HRP Substrate enables detection of target proteins in small amounts.