APC-XFD750 Tandem

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	754
Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	776

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501

Direct upgrades

Overview SDS Protocol

Upgrade: APC-iFluor® 750 Tandem

Absorbance (nm)

754

Extinction coefficient (cm ^-1 M ^-1)

700000

Excitation (nm)

651

Emission (nm)

776

Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). APC-XFD750 tandem has the structure and properties to the commonly used APC-Alexa Fluor® 750. Its primary absorption peak is at 651 nm with emission peak at ~780 nm.

Spectrum

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Spectral properties

Absorbance (nm)	754
Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	776

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
APC-Cy7 Tandem	651	779	700000
APC-XFD700 Tandem	651	707	700000

Images

Figure 1. Flow cytometry analysis of PBMC stained with APC-XFD750 anti-human CD3 *SK7* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-XFD750 specific R7-A channel. XFD750 is the same structure as Alexa Fluor® 750.

Figure 2. Flow cytometry analysis of whole blood stained with APC-XFD750 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-XFD750 specific R7-A channel. XFD750 is the same structure as Alexa Fluor® 750.

References

View all 5 references: Citation Explorer

Comparison between photostability of Alexa Fluor 448 and Alexa Fluor 647 with conventional dyes FITC and APC by flow cytometry.
Authors: Rai, S and Bhardwaj, U and Misra, A and Singh, S and Gupta, R
Journal: International journal of laboratory hematology (2018): e52-e54

Dual-Color Fluorescence Imaging of EpCAM and EGFR in Breast Cancer Cells with a Bull's Eye-Type Plasmonic Chip.
Authors: Izumi, Shota and Yamamura, Shohei and Hayashi, Naoko and Toma, Mana and Tawa, Keiko
Journal: Sensors (Basel, Switzerland) (2017)

[Changes of monocyte and monocyte-platelet aggregates in different subgroups of thrombotic events in patients with acute myocardial infarction during PCI].
Authors: Wang, Sheng and Sun, Cuifang and Liao, Wang and Wu, Zhongwei and Wang, Yudai and Huang, Xiuxian and Lu, Sijia and Dong, Xiaoli and Shuai, Fujie and Li, Bin
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2017): 959-965

Antigen processing of glycoconjugate vaccines; the polysaccharide portion of the pneumococcal CRM(197) conjugate vaccine co-localizes with MHC II on the antigen processing cell surface.
Authors: Lai, Zengzu and Schreiber, John R
Journal: Vaccine (2009): 3137-44

Investigating the role of Natural Killer T-cells in Gram negative infections of patients with type 2 diabetes mellitus.
Authors: Karagianni, P and Polyzos, S A and Bougiouklis, D and Tsapas, A and Paletas, K
Journal: Hippokratia : 231-4