ATTO 532 NHS ester is manufactured by AAT Bioquest for research and development use. ATTO 532 is a rhodamine-based fluorescent dye characterized by its strong absorption and exceptional fluorescence quantum yield (0.90). It demonstrates good photostability along with excellent water solubility, and features a sufficient Stokes shift (Ex/Em = 531/552). ATTO 532 is highly suitable for single-molecule detection and high-resolution microscopy techniques such as SIM and STED. Additionally, it is well-suited for flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a variety of other biological assays, making it a versatile tool in advanced fluorescence-based research. It is optimally excited within the 515-545 nm range, with the 532 nm output of a frequency-doubled Nd laser serving as an ideal excitation source.

NHS esters react selectively and efficiently with primary amines (such as the side chains of lysine residues or aminosilane-coated surfaces) at pH 7-9, forming stable covalent amide bonds. This property makes ATTO 532 NHS ester an excellent choice for labeling peptides and amine-modified oligonucleotides. For labeling antibodies and other proteins, we highly recommend our iFluor® dyes that are highly water soluble and are optimized for labeling proteins.