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Telephone:1-408-733-1055
Fax:1-408-733-1304
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Purchase Order:sales@aatbio.com

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ATTO 565 Acid, 5-Isomer

Product key features

  • Ex/Em: 562/589 nm
  • Extinction coefficient: 120,000 cm-1M-1
  • High Quantum Yield & Photostability: Delivers strong, stable fluorescence for sensitive applications
  • Enhanced Signal Separation: Provides effective signal clarity by minimizing background interference
  • Ideal for Super-Resolution Imaging: Suited for PALM, dSTORM, and STED techniques in high-resolution microscopy

Product description

ATTO 565 Acid, 5-Isomer is manufactured by AAT Bioquest for research and development use. ATTO 565 Acid, 5-Isomer is an amine-reactive rhodamine dye commonly used for labeling peptides, proteins, and other amine-containing molecules, such as amino-modified oligonucleotides. In the presence of a coupling reagent such as EDC, this dye reacts with amine groups to form a stable amide bond. ATTO 565 Acid, 5-Isomer demonstrates strong absorption, high fluorescence quantum yield, and good thermal and photostability. However, it has low water solubility. For applications requiring high water solubility, iFluor® 568 acid is recommended as an alternative.

Spectrum

References

View all 7 references: Citation Explorer
The new live imagers MitoMM1/2 for mitochondrial visualization.
Authors: Maeda, Miwa and Suzuki, Mayu and Takashima, Shigeo and Sasaki, Tsutomu and Oh-Hashi, Kentaro and Takemori, Hiroshi
Journal: Biochemical and biophysical research communications (2021): 50-54
DNA-templated control of chirality and efficient energy transport in supramolecular DNA architectures with aggregation-induced emission.
Authors: Ucar, Hülya and Wagenknecht, Hans-Achim
Journal: Chemical science (2021): 10048-10053
Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter.
Authors: Mathiassen, Patricia P M and Menon, Anant K and Pomorski, Thomas Günther
Journal: Scientific reports (2021): 14364
Importance of probe design for bioanalysis of oligonucleotides using hybridization-based LC-fluorescence assays.
Authors: Ji, Yuhuan and Liu, Yijiang and Xia, Wanhong and Behling, Alexander and Meng, Min and Bennett, Patrick and Wang, Laixin
Journal: Bioanalysis (2019): 1917-1925
The effect of local dynamics of Atto 390-labeled lysozyme on fluorescence anisotropy modeling.
Authors: Babcock, Jeremiah J and Brancaleon, Lorenzo
Journal: Biopolymers (2015): 285-95
Page updated on June 5, 2025

Ordering information

Price
Unit size
Catalog Number2826
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
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Physical properties

Molecular weight

624.61

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.27

Correction Factor (280 nm)

0.12

Extinction coefficient (cm -1 M -1)

120000

Excitation (nm)

562

Emission (nm)

589

Quantum yield

0.90

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
With EDAC or other equivalent activating coupling agents, fluorescent dyes, such as ATTO 565 Acid, 5-Isomer, can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes, such as ATTO 565 Acid, 5-Isomer, can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes, such as ATTO 565 Acid, 5-Isomer, can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.

Documents

pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
Peptide and Oligonucleotide Labeling