Name: AzoDye-1 Phosphoramidite
Brand: AAT Bioquest
SKU: 2416
Availability: InStock

AzoDye-1 Phosphoramidite

AzoDye-1 Phosphoramidite is a crucial reagent widely used in synthesizing oligonucleotides with numerous biomedical applications. The oligos prepared from AzoDye-1 are widely used in qPCR-based nucleic acid detections. AzoDye-1 is a key member of the commonly used robust dark quenchers that exhibit excellent quenching efficiencies, making it valuable for developing fluorescence resonance energy transfer (FRET) probes. These probes are essential tools in molecular biology research, allowing scientists to study DNA and RNA interactions, gene expression, and other biological processes.

Datasheet

Catalog	Size	Price	Quantity
2416	50 umoles	Price
2417	100 umoles	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-1 Phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	147.763 µL	738.814 µL	1.478 mL	7.388 mL	14.776 mL
5 mM	29.553 µL	147.763 µL	295.526 µL	1.478 mL	2.955 mL
10 mM	14.776 µL	73.881 µL	147.763 µL	738.814 µL	1.478 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	676.76 g/mol	=	mL	x	mM	=	mmol

References

View all 35 references: Citation Explorer

Sensitive fluorescence biosensor for SARS-CoV-2 nucleocapsid protein detection in cold-chain food products based on DNA circuit and g-CNQDs@Zn-MOF.

Authors:

Zhou, Chen and Lin, Chiliang and Hu, Yuyao and Zan, Haocheng and Xu, Xiaruiyan and Sun, Chengjun and Zou, Haimin and Li, Yongxin

Journal:

Lebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire (2022): 114032

Enzymatic Beacons for Specific Sensing of Dilute Nucleic Acid.

Authors:

Zhang, Xiaoyu and Kotikam, Venubabu and Rozners, Eriks and Callahan, Brian P

Journal:

Chembiochem : a European journal of chemical biology (2022): e202100594

An Advanced Detection System for In Situ Hybridization Using a Fluorescence Resonance Energy Transfer-based Molecular Beacon Probe.

Authors:

Choijookhuu, Narantsog and Shibata, Yasuaki and Ishizuka, Takumi and Xu, Yan and Koji, Takehiko and Hishikawa, Yoshitaka

Journal:

Acta histochemica et cytochemica (2022): 119-128

Enzymatic Beacons for Specific Sensing of Dilute Nucleic Acid and Potential Utility for SARS-CoV-2 Detection.

Authors:

Zhang, Xiaoyu and Kotikam, Venubabu and Rozners, Eriks and Callahan, Brian P

Journal:

bioRxiv : the preprint server for biology (2021)

A Peptide-PNA Hybrid Beacon for Sensitive Detection of Protein Biomarkers in Biological Fluids.

Authors:

Dhar, Amlanjyoti and Ahmed, Israr and Mallick, Shampa and Roy, Siddhartha

Journal:

Chembiochem : a European journal of chemical biology (2020): 2121-2125

Physical properties

Molecular weight	676.76
Solvent	DMSO

Spectral properties

Absorbance (nm)	534
Extinction coefficient (cm ^-1 M ^-1)	34000

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	1430816-77-5

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Page updated on April 16, 2026