BCECF, AM | AAT Bioquest

BCECF, AM

Intracellular pH plays an important modulating role in many cellular events, including cell growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, cell adhesion and other cellular processes. pH-sensitive fluorescent dyes have been widely applied to monitor changes in intracellular pH in recent years. Imaging techniques that use fluorescent pH indicators also allow researchers to investigate these processes with much greater spatial resolution and sampling density that can be achieved using other technologies such as microelectrode. Among them, 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) is the most popular pH probe since it can be used to monitor cellular pH ratiometrically. BCECF AM is the cell-permeable version of BCECF.

Catalog	Size	Price	Quantity
21202	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of BCECF, AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	145.224 µL	726.121 µL	1.452 mL	7.261 mL	14.522 mL
5 mM	29.045 µL	145.224 µL	290.449 µL	1.452 mL	2.904 mL
10 mM	14.522 µL	72.612 µL	145.224 µL	726.121 µL	1.452 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	688.59 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

The F0F1 ATP synthase regulates human neutrophil migration through cytoplasmic proton extrusion coupled with ATP generation

Authors:

Gao, Jun and Zhang, Tian and Kang, Zhanfang and Ting, Weijen and Xu, Lingqing and Yin, Dazhong

Journal:

Molecular Immunology (2017): 219--226

Oxidative Stress-Activated NHE1 Is Involved in High Glucose-Induced Apoptosis in Renal Tubular Epithelial Cells

Authors:

Wu, Yiqing and Zhang, Min and Liu, Rui and Zhao, Chunjie

Journal:

Yonsei Medical Journal (2016): 1252--1259

References

View all 34 references: Citation Explorer

Simultaneous measurement of water volume and pH in single cells using BCECF and fluorescence imaging microscopy

Authors:

Alvarez-Leefmans FJ, Herrera-Perez JJ, Marquez MS, Blanco VM.

Journal:

Biophys J (2006): 608

Photophysics of the fluorescent pH indicator BCECF

Authors:

Boens N, Qin W, Basaric N, Orte A, Talavera EM, Alvarez-Pez JM.

Journal:

J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 9334

Drug efflux transport properties of 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (bcecf-am) and its fluorescent free acid, bcecf

Authors:

Bachmeier CJ, Trickler WJ, Miller DW.

Journal:

J Pharm Sci (2004): 932

A rapid method for measuring intracellular pH using BCECF-AM

Authors:

Ozkan P, Mutharasan R.

Journal:

Biochim Biophys Acta (2002): 143

Detection of MRP functional activity: calcein AM but not BCECF AM as a Multidrug Resistance-related Protein (MRP1) substrate

Authors:

Olson DP, Taylor BJ, Ivy SP.

Journal:

Cytometry (2001): 105

Physical properties

Molecular weight	688.59
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	504
Emission (nm)	527

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	117464-70-7

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	490, 430
Emission	535
Cutoff	515
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

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Page updated on October 2, 2025