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Biotin-14-dCTP *1 mM in Tris Buffer (pH 7.5)*

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Physical properties
Molecular weight905.79
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134
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OverviewpdfSDSpdfProtocol


Molecular weight
905.79
The biotin-modified dCTP analogs are widely used for a variety of non-radioactive DNA labeling reactions including nick translation, random prime labeling, cDNA labeling and 3'-end labeling. The biotinylated probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probes can be detected by avidin and streptavidin. Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as substitute for dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. The 14-atom spacer between the N4 position of cytosine and biotin tag seperates the CTP base from the biotin tag ensuring the optimal properties for Nick Translation. For PCR incorporation experiments with Taq polymerase, 50% Biotin-14-dCTP/ 50% dCTP ratio might be used for Nick Translation. Alternatively, you might determine the best ratio under your test conditions.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Biotin-14-dCTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM110.401 µL552.004 µL1.104 mL5.52 mL11.04 mL
5 mM22.08 µL110.401 µL220.802 µL1.104 mL2.208 mL
10 mM11.04 µL55.2 µL110.401 µL552.004 µL1.104 mL

Molarity calculator

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Citations


View all 2 citations: Citation Explorer
Global loss of fine-scale chromatin architecture and rebalancing of gene expression during early colorectal cancer development
Authors: Snyder, Michael and Zhu, Yizhou and Lee, Hayan and Weimer, Annika and Horning, Aaron and Nevins, Stephanie and Esplin, Edward and Paul, Kristina and Krieger, Gat and Shipony, Zohar and others,
Journal: (2022)
Characterizing single-molecule conformational changes under shear flow with fluorescence microscopy
Authors: Pisapati, Avani V and Wang, Yi and Blauch, Megan E and Wittenberg, Nathan J and Cheng, Xuanhong and Zhang, X Frank
Journal: JoVE (Journal of Visualized Experiments) (2020): e60784

References


View all 9 references: Citation Explorer
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229
A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145
Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166
Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953