Biotin-14-dCTP *1 mM in Tris Buffer (pH 7.5)*

Biotin-14-dCTP

1 mM in Tris Buffer (pH 7.5)

The biotin-modified dCTP analogs are widely used for a variety of non-radioactive DNA labeling reactions including nick translation, random prime labeling, cDNA labeling and 3'-end labeling. The biotinylated probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probes can be detected by avidin and streptavidin. Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as substitute for dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. The 14-atom spacer between the N4 position of cytosine and biotin tag seperates the CTP base from the biotin tag ensuring the optimal properties for Nick Translation. For PCR incorporation experiments with Taq polymerase, 50% Biotin-14-dCTP/ 50% dCTP ratio might be used for Nick Translation. Alternatively, you might determine the best ratio under your test conditions.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17019	25 nmoles	Price

Citations

View all 2 citations: Citation Explorer

Global loss of fine-scale chromatin architecture and rebalancing of gene expression during early colorectal cancer development

Authors:

Snyder, Michael and Zhu, Yizhou and Lee, Hayan and Weimer, Annika and Horning, Aaron and Nevins, Stephanie and Esplin, Edward and Paul, Kristina and Krieger, Gat and Shipony, Zohar and others,

Characterizing single-molecule conformational changes under shear flow with fluorescence microscopy

Authors:

Pisapati, Avani V and Wang, Yi and Blauch, Megan E and Wittenberg, Nathan J and Cheng, Xuanhong and Zhang, X Frank

Journal:

JoVE (Journal of Visualized Experiments) (2020): e60784

References

View all 9 references: Citation Explorer

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1

Authors:

Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.

Journal:

Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling

Authors:

Cox WG, Singer VL.

Journal:

Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX

Authors:

Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.

Journal:

Bioconjug Chem (2003): 919

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking

Authors:

Wooddell CI, Burgess RR.

Journal:

Biochemistry (2000): 13405

Simple method for preparation of fluor/hapten-labeled dUTP

Authors:

Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.

Journal:

Biotechniques (2000): 518

Physical properties

Molecular weight	905.79
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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