BrdUTP [5-Bromo-2'-deoxyuridine 5'-triphosphate] *10 mM in TE Buffer* *CAS 102212-99-7*
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Physical properties
Molecular weight | 612.98 |
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Overview | SDSProtocol |
See also: Fluorescent in situ hybridization (FISH)
CAS 102212-99-7 | Molecular weight 612.98 |
BrdUTP can be used for incorporating into DNA for the subsequent detection with anti-BrdU antibodies. BrdUTP incorporation into DNA is also a tool for random-mutation introduction.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of BrdUTP [5-Bromo-2'-deoxyuridine 5'-triphosphate] *10 mM in TE Buffer* *CAS 102212-99-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 163.137 µL | 815.687 µL | 1.631 mL | 8.157 mL | 16.314 mL |
5 mM | 32.627 µL | 163.137 µL | 326.275 µL | 1.631 mL | 3.263 mL |
10 mM | 16.314 µL | 81.569 µL | 163.137 µL | 815.687 µL | 1.631 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Citations
View all 1 citations: Citation Explorer
Heat-shock pre-treatment reduces liver injury and aids liver recovery after partial hepatectomy in mice
Authors: Oka, Yousuke and Akagi, Yoshito and Kinugasa, Tetsushi and Ishibashi, Nobuya and Iwakuma, Nobutaka and Shiratsuchi, Ichitarou and Shirouzu, Kazuo
Journal: Anticancer research (2013): 2887--2894
Authors: Oka, Yousuke and Akagi, Yoshito and Kinugasa, Tetsushi and Ishibashi, Nobuya and Iwakuma, Nobutaka and Shiratsuchi, Ichitarou and Shirouzu, Kazuo
Journal: Anticancer research (2013): 2887--2894
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
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I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?