logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Cell Meter™ Cell Viability Assay Kit
Red Fluorescence
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This kit uses a proprietary non-fluorescent dye that gets enhanced red fluorescence upon entering into live cells. The dye is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the non-fluorescent substrate by intracellular esterases generates a strongly fluorescent hydrophilic product that is well-retained in the cell cytoplasm. The esterase activity is proportional to the number of viable cells, and thus directly related to the fluorescence intensity of the product generated from the esterase-catalyzed hydrolysis of the fluorogenic substrate. Cells grown in black-walled plates can be stained and quantified in less than two hours. The assay is more robust than the tetrazolium salt or Alarmar Blue™-based assays. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. The fluorogenic indicator has spectral properties compatible with Cy3/TRITC filter set.
HeLa cell number response was measured with Cell Meter™ Cell Viability Assay Kit. HeLa cells at 0 to 3,000 cells/well/100 µL were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of CytoCalcein™ Red dye-working solution for 30 minutes at 37°C. The fluorescence intensity was measured at Ex/Em = 540/590 nm (Cutoff = 570 nm) with bottom read mode using Flexstation (from Molecular devices).
HeLa cell number response was measured with Cell Meter™ Cell Viability Assay Kit. HeLa cells at 0 to 3,000 cells/well/100 µL were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of CytoCalcein™ Red dye-working solution for 30 minutes at 37°C. The fluorescence intensity was measured at Ex/Em = 540/590 nm (Cutoff = 570 nm) with bottom read mode using Flexstation (from Molecular devices).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22783200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on October 9, 2025