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Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit
Optimized for Flow Cytometry
Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways. Cell Meter™ Fluorimetric ROS Assay Kit uses our unique Amplite® ROS Green sensor to quantify ROS in live cells. Amplite® ROS Green is cell-permeable. It generates the green fluorescence when it reacts with ROS. The Cell Meter™ Fluorimetric ROS Assay Kit provides a sensitive, one-step fluorimetric assay to detect intracellular ROS in live cells with one hour incubation. This kit is optimized for flow cytometry applications, its signal can be detected with Ex/Em = 490/520 nm (FL1 channel).
Detection of intracellular ROS in Jurkat cells upon TBHP treatment using Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit. Cells were incubated with Amplite® ROS Green at 37 °C for 1 hour. The cells were then treated without (Green) or with (Red) 100 µM TBHP at 37 °C for 30 minutes. The fluorescence signal was monitored at FITC channel using a flow cytometer (Acea NovoCyte 3000).
Detection of intracellular ROS in Jurkat cells upon TBHP treatment using Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit. Cells were incubated with Amplite® ROS Green at 37 °C for 1 hour. The cells were then treated without (Green) or with (Red) 100 µM TBHP at 37 °C for 30 minutes. The fluorescence signal was monitored at FITC channel using a flow cytometer (Acea NovoCyte 3000).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22904100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel
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Page updated on October 6, 2025