Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
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Catalog Number | |
Unit Size | |
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Excitation (nm) | 498 |
Emission (nm) | 517 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Excitation (nm) 498 | Emission (nm) 517 |
Platform
Flow cytometer
Excitation | 488 nm laser |
Emission | 530/30 nm filter |
Instrument specification(s) | FITC channel |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare cells in growth medium
- Stain cells with OxiVision™ Green peroxide sensor
- Treat cells with test compounds
- Monitor fluorescence intensity with flow cytometer FITC channel (Ex/Em = 490/530 nm)
Important notes
Thaw all the four components at room temperature before use.
PREPARATION OF STOCK SOLUTION
1. OxiVision™ Green Peroxide Sensor stock solution:
Add 100 µL of DMSO (Component B) into the vial of OxiVision™ Green Peroxide Sensor (Component A), and mix well. Note: 1 µL of reconstituted OxiVision™ Green Peroxide Sensor stock solution is for 0.5 mL cells. The stock solution should be used promptly. Protect from light.
For guidelines on cell sample preparation, please visit
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
SAMPLE EXPERIMENTAL PROTOCOL
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Stain cells with OxiVision™ Green Peroxide Sensor stock solution in full medium or in your desired buffer at 37°C for 30 minutes, protected from light.
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Treat cells with test compounds in full medium or in your desired buffer at 37°C for desired period of time. For control samples (untreated cells), add the corresponding amount of compound buffer. Note: It is recommended to treat cells in full medium. However, if tested compounds are serum sensitive, growth medium and serum factors can be aspirated away before treatment. Resuspend cells in 1X Hank’s salt solution and 20 mM Hepes buffer (HHBS) or the buffer of your choice after aspiration. Alternatively, cells can be treated in serum-free media. Note: We treated Jurkat cells with 100 µM hydrogen peroxide in full medium at 37°C for 90 minutes to induce hydrogen peroxide. See Figure 1 for details.
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Alternatively, treat cells with tested compounds at 37°C for desired period of time. Remove the treatment solution, then stain cells with OxiVision™ Green Peroxide Sensor stock solution in full medium or in your desired buffer at 37°C for desired period of time.
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Monitor the fluorescence intensity at FITC channel (Ex/Em = 490/530 nm) using a flow cytometer. Gate on the cells of interest, excluding debris.
Images

Citations
Authors: Samarin, Jana and Fabrowski, Piotr and Kurilov, Roman and Nuskova, Hana and Hummel-Eisenbeiss, Johanna and Pink, Hannelore and Li, Nan and Weru, Vivienn and Alborzinia, Hamed and Yildiz, Umut and others,
Journal: bioRxiv (2023): 2023--01
Authors: Hu, Can and Qiu, Yan and Guo, Jiajun and Cao, Yuchao and Li, Dairong and Du, Yonghong
Journal: International Journal of Nanomedicine (2023): 6257--6274
Authors: Ishak, Nur Syafiqah Mohamad and Numaguchi, Tomoe and Ikemoto, Kazuto
Journal: ACS Omega (2023)
Authors: Liu, Huan and Qu, Xue and Tan, Haoqi and Song, Jialin and Lei, Miao and Kim, Eunkyoung and Payne, Gregory F and Liu, Changsheng
Journal: Acta Biomaterialia (2019)
Authors: Matsumoto, Ryo and Shimizu, Kazunori and Nagashima, Takunori and Tanaka, Hiromasa and Mizuno, Masaaki and Kikkawa, Fumitaka and Hori, Masaru and Honda, Hiroyuki
Journal: Regenerative Therapy (2016): 55--63
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Application notes
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