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Cell Meter™ Intracellular NADH/NADPH Flow Cytometric Analysis Kit
Red Fluorescence
The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery. In general, the redox couples NAD/NADH and NADP/NADPH play a critical role in energy metabolism, glycolysis, tricarboxylic acid cycle and mitochondrial respiration. The increased NAD(P)H level in cells is linked to the abnormal production of reactive oxygen species (ROS) and DNA damage. However, due to the lack of sensitive NAD(P)H probe, it has been challenging to detect intracellular NAD(P)H in biological systems. Cell Meter™ Intracellular NADH/NADPH Flow Cytometric Analysis Kit provides an efficient method to monitor intracellular NAD(P)H level in live cells. JZL1707 NAD(P)H sensor has been developed as an excellent fluorescent probe for detecting and imaging NADH/NADPH in cells. The probe bind NADH/NADPH to generate strong fluorescence signal with high sensitivity and specificity. JZL1707 NAD(P)H sensor can be readily loaded into live cells, and its fluorescence signal can be conveniently monitored using flow cytometer in PE channel.
<p>(A) Flow cytometric analysis of NADH/NADPH measurement in Jurkat cells using Cell Meter&trade; Intracellular NADH/NADPH Flow Cytometric Analysis Kit (Cat#15291). Cells were incubated with or without 100 &micro;M NADH in serum-free medium for 30 minutes and then co-incubated with JZL1707 NAD(P)H sensor working solution for another 30 minutes.<br />(B) Fold increase of fluorescence signal intensity of Jurkat cells treated with 100 &micro;M NADH or 100 &micro;M NADPH compared<br />with untreated control. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in PE channel.</p>
<p>(A) Flow cytometric analysis of NADH/NADPH measurement in Jurkat cells using Cell Meter&trade; Intracellular NADH/NADPH Flow Cytometric Analysis Kit (Cat#15291). Cells were incubated with or without 100 &micro;M NADH in serum-free medium for 30 minutes and then co-incubated with JZL1707 NAD(P)H sensor working solution for another 30 minutes.<br />(B) Fold increase of fluorescence signal intensity of Jurkat cells treated with 100 &micro;M NADH or 100 &micro;M NADPH compared<br />with untreated control. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in PE channel.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
15291100 Tests
Price
 
Spectral properties
Excitation (nm)535
Emission (nm)557
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission575/26 nm filter
Instrument specification(s)PE channel
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Page updated on September 30, 2025