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Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit
Blue Fluorescence
The endoplasmic reticulum (ER) is a type of organelle in the cells of eukaryotic organisms that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae. The membranes of the ER are continuous with the outer nuclear membrane. ER occurs in most types of eukaryotic cells, but is absent from red blood cells and spermatozoa. This Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit uses our ER Tracer™ Blue as an ER marker. ER Tracer™ Blue stain is a cell-permeant fluorescent dye that is highly selective for ER. This stain consists of a blue fluorescent dye and ER binder that selectively bind to ER in most of cell types. For some cells, ER Tracer™ Blue may not selectively bind to ER. ER Tracer™ Blue has spectral properties similar to DAPI, making this kit convenient with the DAPI filter set. ER Tracer™ is good replacement for ER Tracker (trademark ThermoFisher).
Fluorescence images of endoplasmic reticulum (ER) staining in HeLa cells cultured in a 96-well black-wall clear-bottom plate using a fluorescence microscope with a DAPI filter set. (A) Live cells were co-stained with ER-selective probe ER Tracer™ Blue (Cat No. 22634, Blue) and Nuclear Red™ LCS1 (Cat No. 17542, Red). (B) Live cells were co-stained with ER-selective probe ER Tracer™ Blue (Cat No. 22634, Blue) and Nuclear Red™ LCS1 (Cat No. 17542, Red) and then were fixed with 4% formaldehyde.
Fluorescence images of endoplasmic reticulum (ER) staining in HeLa cells cultured in a 96-well black-wall clear-bottom plate using a fluorescence microscope with a DAPI filter set. (A) Live cells were co-stained with ER-selective probe ER Tracer™ Blue (Cat No. 22634, Blue) and Nuclear Red™ LCS1 (Cat No. 17542, Red). (B) Live cells were co-stained with ER-selective probe ER Tracer™ Blue (Cat No. 22634, Blue) and Nuclear Red™ LCS1 (Cat No. 17542, Red) and then were fixed with 4% formaldehyde.
protocol
Protocol
sds
SDS
COO
COA
CatalogSize
Price
Quantity
22634100 Tests
Price
 
Spectral properties
Excitation (nm)344
Emission (nm)457
Storage, safety and handling
Intended useResearch Use Only (RUO)
Instrument settings
Fluorescence microscope
ExcitationDAPI filter set
EmissionDAPI filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis
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Page updated on October 30, 2025