CFDA [5-(and 6)-Carboxyfluorescein diacetate] *Mixed isomers*

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Chemical structure for CFDA [5-(and 6)-Carboxyfluorescein diacetate] *Mixed isomers*
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Unit Size: Cat No: Price (USD): Qty:
100 mg 22021 $75

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Ex/Em (nm)494/521
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Category Cell Biology
Labeling Cells
Related Viability and Proliferation
Cell Viability
Secondary Reagents
It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample. Flow cytometry combined with fluorescent staining is a powerful tool to analyze heterogeneous cell populations. Fluorescein diacetate (FDA) and its derivatives are non-fluorescent molecules that diffuse into cells and are hydrolyzed by intracellular non-specific esterases to give fluorescent products. The fluorescent products can be accumulated only in those cells that have intact cell membranes; therefore, dead cells with leaky membranes are not stained. The precise kinetics of membrane transport and intracellular hydrolysis of FDA and its analogs (such as CDCFDA) are related to cellular functions, thus FDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The fluorescence intensity of labeled cells by FDA dyes varies considerably among cell lines and strains, probably because of differences in intracellular esterase activity. CFDA is an amine-reactive FDA derivative that can be used to prepare a variety of FDA conjugates.

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CFDA [5-(and 6)-Carboxyfluorescein diacetate] *Mixed isomers* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =

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Wavelength (nm)

References & Citations

Fluid and cell behaviors along a 3D printed alginate/gelatin/fibrin channel
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Journal: Biotechnology and bioengineering (2015): 1683--1695

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Journal: International journal of molecular sciences (2014): 20101--20116

Identification of VAR2CSA domain-specific inhibitory antibodies of the Plasmodium falciparum erythrocyte membrane protein 1 using a novel flow cytometry assay
Authors: Harold Obiakor, Marion Avril, Nicholas J MacDonald, Prakash Srinivasan, Karine Reiter, Charles Anderson, Kevin L Holmes, Michal Fried, Patrick E Duffy, Joseph D Smith
Journal: Clinical and Vaccine Immunology (2013): 433--442

Aging enhances maceration-induced ultrastructural alteration of the epidermis and impairment of skin barrier function
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