Coelenterazine h *CAS#: 50909-86-9*
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Additional ordering information
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Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 407.46 |
Solvent | Ethanol |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Intracellular Ions
CAS 50909-86-9 | Molecular weight 407.46 |
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin's bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 µM to >100 µM. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10-20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Calculators
Common stock solution preparation
Table 1. Volume of Ethanol needed to reconstitute specific mass of Coelenterazine h *CAS#: 50909-86-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 245.423 µL | 1.227 mL | 2.454 mL | 12.271 mL | 24.542 mL |
5 mM | 49.085 µL | 245.423 µL | 490.846 µL | 2.454 mL | 4.908 mL |
10 mM | 24.542 µL | 122.711 µL | 245.423 µL | 1.227 mL | 2.454 mL |
Molarity calculator
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Citations
View all 11 citations: Citation Explorer
Remdesivir inhibits Porcine epidemic diarrhea virus infection in vitro
Authors: Huang, Zi-Xin and Zhou, Shu-Ting and Wang, Jing and Yang, Zhi-Biao and Wang, Zhe
Journal: Heliyon (2023)
Authors: Huang, Zi-Xin and Zhou, Shu-Ting and Wang, Jing and Yang, Zhi-Biao and Wang, Zhe
Journal: Heliyon (2023)
Molnupiravir Inhibits Porcine Epidemic Diarrhea Virus Infection In Vitro
Authors: Huang, Zi-Xin and Zhou, Shu-Ting and Yang, Zhi-Biao and Wang, Zhe
Journal: Viruses (2023): 1317
Authors: Huang, Zi-Xin and Zhou, Shu-Ting and Yang, Zhi-Biao and Wang, Zhe
Journal: Viruses (2023): 1317
Wounding induces a peroxisomal H2O2 decrease via glycolate oxidase-catalase switch dependent on glutamate receptor-like channel-supported Ca2+ signaling in plants
Authors: Li, Xiangyang and Chen, Linru and Zeng, Xiaoyue and Wu, Kaixin and Huang, Jiayu and Liao, Mengmeng and Xi, Yue and Zhu, Guohui and Zeng, Xiuying and Hou, Xuewen and others,
Journal: The Plant Journal (2023)
Authors: Li, Xiangyang and Chen, Linru and Zeng, Xiaoyue and Wu, Kaixin and Huang, Jiayu and Liao, Mengmeng and Xi, Yue and Zhu, Guohui and Zeng, Xiuying and Hou, Xuewen and others,
Journal: The Plant Journal (2023)
Elucidation of the molecular and cellular mechanisms of FKBP51-selective inhibitors
Authors: Haehle, Andreas
Journal: (2020)
Authors: Haehle, Andreas
Journal: (2020)
Split Nano Luciferase Complementation for Probing Protein-Protein Interactions in Plant Cells
Authors: Wang, Feng-Zhu and Zhang, Nannan and Guo, Yan-Jun and Gong, Ben-Qiang and Li, Jian-Feng
Journal: Journal of integrative plant biology (2019)
Authors: Wang, Feng-Zhu and Zhang, Nannan and Guo, Yan-Jun and Gong, Ben-Qiang and Li, Jian-Feng
Journal: Journal of integrative plant biology (2019)
Molecular characterization of neuropeptide elevenin and two elevenin receptors, IsElevR1 and IsElevR2, from the blacklegged tick, Ixodes scapularis
Authors: Kim, Donghun and Simo, Ladislav and Park, Yoonseong
Journal: Insect Biochemistry and Molecular Biology (2018)
Authors: Kim, Donghun and Simo, Ladislav and Park, Yoonseong
Journal: Insect Biochemistry and Molecular Biology (2018)
Intracellular Ca2+ is important for flagellin-triggered defense in Arabidopsis and involves inositol polyphosphate signaling
Authors: Ma, Yi and Zhao, Yichen and Berkowitz, Gerald A
Journal: Journal of Experimental Botany (2017)
Authors: Ma, Yi and Zhao, Yichen and Berkowitz, Gerald A
Journal: Journal of Experimental Botany (2017)
Bioluminescence resonance energy transfer (BRET) assay for determination of molecular interactions in living cells
Authors: Harikumar, Kaleeckal G and Yan, Yan and Xu, Ting-Hai and Melcher, Karsten and Xu, H Eric and Miller, Laurence J
Journal: Bio-protocol (2017)
Authors: Harikumar, Kaleeckal G and Yan, Yan and Xu, Ting-Hai and Melcher, Karsten and Xu, H Eric and Miller, Laurence J
Journal: Bio-protocol (2017)
Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells
Authors: Harikumar, Kaleeckal G and Yan, Yan and Xu, Ting-Hai and Melcher, Karsten and Xu, H Eric and Miller, Laurence J
Journal: (2017)
Authors: Harikumar, Kaleeckal G and Yan, Yan and Xu, Ting-Hai and Melcher, Karsten and Xu, H Eric and Miller, Laurence J
Journal: (2017)
Molecular physiology of tick salivary secretion and transcriptomics of tick in interaction with tick-borne pathogen
Authors: Kim, Donghun
Journal: (2016)
Authors: Kim, Donghun
Journal: (2016)
References
View all 150 references: Citation Explorer
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Authors: Teranishi K., undefined
Journal: Bioorg Chem. (2006)
Authors: Teranishi K., undefined
Journal: Bioorg Chem. (2006)
Extended bioluminescence resonance energy transfer (eBRET) for monitoring prolonged protein-protein interactions in live cells
Authors: Pfleger KD, Dromey JR, Dalrymple MB, Lim EM, Thomas WG, Eidne KA.
Journal: Cell Signal (2006): 1664
Authors: Pfleger KD, Dromey JR, Dalrymple MB, Lim EM, Thomas WG, Eidne KA.
Journal: Cell Signal (2006): 1664
Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state
Authors: Liu ZJ, Stepanyuk GA, Vysotski ES, Lee J, Markova SV, Malikova NP, Wang BC.
Journal: Proc Natl Acad Sci U S A (2006): 2570
Authors: Liu ZJ, Stepanyuk GA, Vysotski ES, Lee J, Markova SV, Malikova NP, Wang BC.
Journal: Proc Natl Acad Sci U S A (2006): 2570
In vivo testing of Renilla luciferase substrate analogs in an orthotopic murine model of human glioblastoma
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Journal: Int J Dev Biol (2006): 561
Self-illuminating quantum dots light the way
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Journal: Nat Biotechnol (2006): 326
Blue fluorescent protein from the calcium-sensitive photoprotein aequorin: catalytic properties for the oxidation of coelenterazine as an oxygenase
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Journal: FEBS Lett (2006): 1977
Authors: Inouye S, Sasaki S.
Journal: FEBS Lett (2006): 1977
Mitochondrial adaptations within chronically ischemic swine myocardium
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Authors: McFalls EO, Sluiter W, Schoonderwoerd K, Manintveld OC, Lamers JM, Bezstarosti K, van Beusekom HM, Sikora J, Ward HB, Merkus D, Duncker DJ.
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In vivo RNA interference-mediated ablation of MDR1 P-glycoprotein
Authors: Pichler A, Zelcer N, Prior JL, Kuil AJ, Piwnica-Worms D.
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Application notes
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
Novel Red Fluorescent Calcium Probes for Functional Analysis of GPCRs and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
Novel Red Fluorescent Calcium Probes for Functional Analysis of GPCRs and Calcium Channel Targets
FAQ
Can you provide a brief protocol on how to use coelenterazine h for small animal in vivo imaging?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
How do you measure Ca2+ response with calcium indicators like Calbryte 520, potassium salt?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
How do you measure Ca2+ response with calcium indicators like Calbryte 520, potassium salt?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?