logo
AAT Bioquest

Coelenterazine h *CAS#: 50909-86-9*

Product Image
Product Image
Gallery Image 1
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight407.46
SolventEthanol
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


CAS
50909-86-9
Molecular weight
407.46
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin's bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 µM to >100 µM. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10-20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.

Calculators


Common stock solution preparation

Table 1. Volume of Ethanol needed to reconstitute specific mass of Coelenterazine h *CAS#: 50909-86-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM245.423 µL1.227 mL2.454 mL12.271 mL24.542 mL
5 mM49.085 µL245.423 µL490.846 µL2.454 mL4.908 mL
10 mM24.542 µL122.711 µL245.423 µL1.227 mL2.454 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Images


Citations


View all 11 citations: Citation Explorer
Remdesivir inhibits Porcine epidemic diarrhea virus infection in vitro
Authors: Huang, Zi-Xin and Zhou, Shu-Ting and Wang, Jing and Yang, Zhi-Biao and Wang, Zhe
Journal: Heliyon (2023)
Molnupiravir Inhibits Porcine Epidemic Diarrhea Virus Infection In Vitro
Authors: Huang, Zi-Xin and Zhou, Shu-Ting and Yang, Zhi-Biao and Wang, Zhe
Journal: Viruses (2023): 1317
Wounding induces a peroxisomal H2O2 decrease via glycolate oxidase-catalase switch dependent on glutamate receptor-like channel-supported Ca2+ signaling in plants
Authors: Li, Xiangyang and Chen, Linru and Zeng, Xiaoyue and Wu, Kaixin and Huang, Jiayu and Liao, Mengmeng and Xi, Yue and Zhu, Guohui and Zeng, Xiuying and Hou, Xuewen and others,
Journal: The Plant Journal (2023)
Split Nano Luciferase Complementation for Probing Protein-Protein Interactions in Plant Cells
Authors: Wang, Feng-Zhu and Zhang, Nannan and Guo, Yan-Jun and Gong, Ben-Qiang and Li, Jian-Feng
Journal: Journal of integrative plant biology (2019)
Molecular characterization of neuropeptide elevenin and two elevenin receptors, IsElevR1 and IsElevR2, from the blacklegged tick, Ixodes scapularis
Authors: Kim, Donghun and Simo, Ladislav and Park, Yoonseong
Journal: Insect Biochemistry and Molecular Biology (2018)
Intracellular Ca2+ is important for flagellin-triggered defense in Arabidopsis and involves inositol polyphosphate signaling
Authors: Ma, Yi and Zhao, Yichen and Berkowitz, Gerald A
Journal: Journal of Experimental Botany (2017)
Bioluminescence resonance energy transfer (BRET) assay for determination of molecular interactions in living cells
Authors: Harikumar, Kaleeckal G and Yan, Yan and Xu, Ting-Hai and Melcher, Karsten and Xu, H Eric and Miller, Laurence J
Journal: Bio-protocol (2017)
Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells
Authors: Harikumar, Kaleeckal G and Yan, Yan and Xu, Ting-Hai and Melcher, Karsten and Xu, H Eric and Miller, Laurence J
Journal: (2017)
Molecular physiology of tick salivary secretion and transcriptomics of tick in interaction with tick-borne pathogen
Authors: Kim, Donghun
Journal: (2016)

References


View all 150 references: Citation Explorer
Luminescence of imidazo[1,2-a]pyrazin-3(7H)-one compounds
Authors: Teranishi K., undefined
Journal: Bioorg Chem. (2006)
Extended bioluminescence resonance energy transfer (eBRET) for monitoring prolonged protein-protein interactions in live cells
Authors: Pfleger KD, Dromey JR, Dalrymple MB, Lim EM, Thomas WG, Eidne KA.
Journal: Cell Signal (2006): 1664
Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state
Authors: Liu ZJ, Stepanyuk GA, Vysotski ES, Lee J, Markova SV, Malikova NP, Wang BC.
Journal: Proc Natl Acad Sci U S A (2006): 2570
In vivo testing of Renilla luciferase substrate analogs in an orthotopic murine model of human glioblastoma
Authors: Otto-Duessel M, Khankaldyyan V, Gonzalez-Gomez I, Jensen MC, Laug WE, Rosol M.
Journal: Mol Imaging (2006): 57
Expression, purification and characterization of calcium-triggered luciferin-binding protein of Renilla reniformis
Authors: Inouye S., undefined
Journal: Protein Expr Purif. (2006)
Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development
Authors: Cheung CY, Webb SE, Meng A, Miller AL.
Journal: Int J Dev Biol (2006): 561
Self-illuminating quantum dots light the way
Authors: Frangioni JV., undefined
Journal: Nat Biotechnol (2006): 326
Blue fluorescent protein from the calcium-sensitive photoprotein aequorin: catalytic properties for the oxidation of coelenterazine as an oxygenase
Authors: Inouye S, Sasaki S.
Journal: FEBS Lett (2006): 1977
Mitochondrial adaptations within chronically ischemic swine myocardium
Authors: McFalls EO, Sluiter W, Schoonderwoerd K, Manintveld OC, Lamers JM, Bezstarosti K, van Beusekom HM, Sikora J, Ward HB, Merkus D, Duncker DJ.
Journal: J Mol Cell Cardiol (2006): 980
In vivo RNA interference-mediated ablation of MDR1 P-glycoprotein
Authors: Pichler A, Zelcer N, Prior JL, Kuil AJ, Piwnica-Worms D.
Journal: Clin Cancer Res (2005): 4487