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Covidyte™ TF670
Coronaviruses (CoVs) can infect humans and multiple species of animals, causing a wide spectrum of diseases. In late 2019, a novel coronavirus, termed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was determined as a cause for several cases of respiratory disease (Covid-19). The virus rapidly spread worldwide. As of May 31st, 2022, more than 6.2 million people have died from coronavirus worldwide, and ~530 million cases have been reported. Currently, there are not any specific and effective options available for treating Covid-19. At present the clinical treatment of Covid-19 is mainly symptomatic combined with repurposing of already marketed antiviral drugs such as Remdesivir and antibiotics to treat secondary infections. There is an extremely urgent need for the development of specific antiviral therapeutics and vaccines against SARS-CoV-2. The coronavirus main protease, which plays a pivotal role in viral gene expression and replication through the proteolytic processing of replicase polyproteins, is an attractive target for anti-CoV drug design. The inhibition of viral proteases necessary for proteolytic processing of polyproteins has been a successful strategy in the treatment of human immunodeficiency virus (HIV) and hepatitis C respectively, proving the potential of protease inhibitors for the treatment of viral infections. Similarly, the main protease of SARS-CoV-2 is thought to be essential for viral replication and, therefore, is regarded as promising target for antiviral therapy of Covid-19. Covidyte™ TF670 is a peptide substrate containing 14 amino acid sequence (KTSAVLQSGFRKME) that can be cleaved by coronavirus proteases. The dark-FRET peptide contains Tide Quencher™ 5 (TQ5) as a quencher and Tide Fluor™ 5 (TF5) as a fluorescent donor on the N-and C-terminals respectively where the fluorescence of TF5 is effectively quenched by TQ5 when the peptide is intact. When the peptide is hydrolyzed by coronavirus proteases, the TF5 fragment generates significantly enhanced fluorescence since its fluorescence is no longer quenched by TQ5. The activity of coronavirus proteases can be effectively monitored by the fluorescence intensity of TF5. Covidyte™ TF670 is a robust high throughput screening tool for searching inhibitors of coronavirus proteases. TQ5-TF5 pair has been proven to an extremely effective FRET pair for developing FRET protease substrates. Comparing to the commonly used EDANS substrates (such as Covidyte™ EN450), the TF670 substrate has much stronger and longer fluorescence that is less interfered by colored compounds that often cause false positive hits.
Proteases play essential roles in protein activation, cell regulation and signaling, as well as in the generation of amino acids for protein synthesis or utilization in other metabolic pathways. FRET protease substrates are widely used for detecting protease activities, in particular, for virus protease that often require a long peptide sequence for optimal binding such as coronavirus, HIV and HCV proteases. The internally quenched FRET peptide substrate is digested by a protease to generate the highly fluorescent peptide fragment. The fluorescence increase is proportional to the protease activity. Tide Quencher™ dyes have been proven to be the extremely effective quenchers for developing FRET protease substrates for high throughput screening applications together with our bright Tide Fluor™ and iFluor® dyes.
Proteases play essential roles in protein activation, cell regulation and signaling, as well as in the generation of amino acids for protein synthesis or utilization in other metabolic pathways. FRET protease substrates are widely used for detecting protease activities, in particular, for virus protease that often require a long peptide sequence for optimal binding such as coronavirus, HIV and HCV proteases. The internally quenched FRET peptide substrate is digested by a protease to generate the highly fluorescent peptide fragment. The fluorescence increase is proportional to the protease activity. Tide Quencher™ dyes have been proven to be the extremely effective quenchers for developing FRET protease substrates for high throughput screening applications together with our bright Tide Fluor™ and iFluor® dyes.
protocol
Protocol
COA
CatalogSize
Price
Quantity
13540100 tests
Price
135411000 tests
Price
 
Physical properties
Molecular weight3620.33
SolventDMSO
Spectral properties
Correction factor (280 nm)0.027
Extinction coefficient (cm -1 M -1)
250000
Excitation (nm)649
Emission (nm)663
Quantum yield
0.27
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Fluorescence microplate reader
Excitation640 nm
Emission680 nm
Cutoff660 nm
Recommended plateSolid black
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Page updated on October 9, 2025