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Cresyl violet *CAS 41830-80-2*

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Physical properties
Molecular weight361.74
Spectral properties
Absorbance (nm)592
Extinction coefficient (cm -1 M -1)83000
Excitation (nm)598
Emission (nm)621
Quantum yield0.54001
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Absorbance (nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
It is used for staining Nissl substances in nerve cells and for bulk staining of nerve tissue. It is also used for preparing Magic Red-based protease substrates that have red fluorescence upon enzyme hydrolysis.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cresyl violet *CAS 41830-80-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM276.442 µL1.382 mL2.764 mL13.822 mL27.644 mL
5 mM55.288 µL276.442 µL552.883 µL2.764 mL5.529 mL
10 mM27.644 µL138.221 µL276.442 µL1.382 mL2.764 mL

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Spectral properties

Absorbance (nm)592
Extinction coefficient (cm -1 M -1)83000
Excitation (nm)598
Emission (nm)621
Quantum yield0.54001



View all 37 references: Citation Explorer
Simultaneous confocal laser endomicroscopy and chromoendoscopy with topical cresyl violet
Authors: Goetz M, Toermer T, Vieth M, Dunbar K, Hoffman A, Galle PR, Neurath MF, Delaney P, Kiesslich R.
Journal: Gastrointest Endosc (2009): 959
Single-molecule fluorescence spectroelectrochemistry of cresyl violet
Authors: Lei C, Hu D, Ackerman EJ.
Journal: Chem Commun (Camb) (2008): 5490
Electronic absorption spectra of cresyl violet acetate in anisotropic and isotropic solvents
Authors: Jafari A, Ghanadzadeh A, Tajalli H, Yeganeh M, Moghadam M.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2007): 717
Chromoendoscopy in Barrett's oesophagus: is cresyl violet the magic bullet
Authors: Kiesslich R, Neurath MF.
Journal: Dig Liver Dis (2006): 301
Infarct volume quantification in mouse focal cerebral ischemia: a comparison of triphenyltetrazolium chloride and cresyl violet staining techniques
Authors: Tureyen K, Vemuganti R, Sailor KA, Dempsey RJ.
Journal: J Neurosci Methods (2004): 203
Cresyl violet as a fluorophore in confocal laser scanning microscopy for future in-vivo histopathology
Authors: George M, Meining A.
Journal: Endoscopy (2003): 585
Fluorogenic substrate [Ala-Pro]2-cresyl violet but not Ala-Pro-rhodamine 110 is cleaved specifically by DPPIV activity: a study in living Jurkat cells and CD26/DPPIV-transfected Jurkat cells
Authors: Boonacker E, Elferink S, Bardai A, Fleischer B, Van Noorden CJ.
Journal: J Histochem Cytochem (2003): 959
DEVDase detection in intact apoptotic cells using the cell permeant fluorogenic substrate, (z-DEVD)2-cresyl violet
Authors: Lee BW, Johnson GL, Hed SA, Darzynkiewicz Z, Talhouk JW, Mehrotra S.
Journal: Biotechniques (2003): 1080
Ala-Pro-cresyl violet, a synthetic fluorogenic substrate for the analysis of kinetic parameters of dipeptidyl peptidase IV (CD26) in individual living rat hepatocytes
Authors: Van Noorden CJ, Boonacker E, Bissell ER, Meijer AJ, van Marle J, Smith RE.
Journal: Anal Biochem (1997): 71
Cresyl violet: a red fluorescent Nissl stain
Authors: Alvarez-Buylla A, Ling CY, Kirn JR.
Journal: J Neurosci Methods (1990): 129