CYTO-13
CYTO-13 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. CYTO-13 has its green fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of live cells with proper filter sets. Based on our in-house evaluations, it has equivalent DNA staining properties almost identical to SYTO-13™ DNA stain (SYTO-13™ is the trade mark of ThermoFisher).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CYTO-13 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 190.687 µL | 953.434 µL | 1.907 mL | 9.534 mL | 19.069 mL |
5 mM | 38.137 µL | 190.687 µL | 381.374 µL | 1.907 mL | 3.814 mL |
10 mM | 19.069 µL | 95.343 µL | 190.687 µL | 953.434 µL | 1.907 mL |
Molarity calculator
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Spectrum
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Page updated on October 8, 2024