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CYTO-13

CYTO-13 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. CYTO-13 has its green fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of live cells with proper filter sets. Based on our in-house evaluations, it has equivalent DNA staining properties almost identical to SYTO-13™ DNA stain (SYTO-13™ is the trade mark of ThermoFisher).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CYTO-13 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM190.687 µL953.434 µL1.907 mL9.534 mL19.069 mL
5 mM38.137 µL190.687 µL381.374 µL1.907 mL3.814 mL
10 mM19.069 µL95.343 µL190.687 µL953.434 µL1.907 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Page updated on October 8, 2024

Ordering information

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Catalog Number17554
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Physical properties

Molecular weight

524.42

Solvent

DMSO

Spectral properties

Excitation (nm)

486

Emission (nm)

511

Storage, safety and handling

Intended useResearch Use Only (RUO)

Storage

Freeze (< -15 °C); Minimize light exposure

Platform

Fluorescence microscope

ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall, clear bottom
Instrument specification(s)FITC filter set
Images of HeLa cells stained with CYO-13 at 5 uM (Left) and 2.5 uM (Right) for 20 minutes at 37&deg;C incubator. Images were acquired using fluorescence microscope with FITC filter
Images of HeLa cells stained with CYO-13 at 5 uM (Left) and 2.5 uM (Right) for 20 minutes at 37&deg;C incubator. Images were acquired using fluorescence microscope with FITC filter
Images of HeLa cells stained with CYO-13 at 5 uM (Left) and 2.5 uM (Right) for 20 minutes at 37&deg;C incubator. Images were acquired using fluorescence microscope with FITC filter

Alternative formats