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CYTO-13
CYTO-13 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. CYTO-13 has its green fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of live cells with proper filter sets. Based on our in-house evaluations, it has equivalent DNA staining properties almost identical to SYTO-13™ DNA stain (SYTO-13™ is the trade mark of ThermoFisher).
Images of HeLa cells stained with CYO-13 at 5 uM (Left) and 2.5 uM (Right) for 20 minutes at 37°C incubator. Images were acquired using fluorescence microscope with FITC filter
Images of HeLa cells stained with CYO-13 at 5 uM (Left) and 2.5 uM (Right) for 20 minutes at 37°C incubator. Images were acquired using fluorescence microscope with FITC filter
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
175545 mg
Price
 
Physical properties
Molecular weight524.42
SolventDMSO
Spectral properties
Excitation (nm)486
Emission (nm)511
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
Instrument settings
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
Instrument specification(s)FITC filter set
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 27, 2025