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DABSYL chloride [4-Dimethylaminoazobenzene-4-sulfonyl chloride] *CAS 56512-49-3*

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Physical properties
Molecular weight323.80
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

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CAS
56512-49-3
Molecular weight
323.80
DABSYL is widely used for labeling amino acids, also used as an acceptor for developing FRET-based nucleic acid probes and protease substrates (often paired with EDANS).

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DABSYL chloride [4-Dimethylaminoazobenzene-4-sulfonyl chloride] *CAS 56512-49-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM308.833 µL1.544 mL3.088 mL15.442 mL30.883 mL
5 mM61.767 µL308.833 µL617.665 µL3.088 mL6.177 mL
10 mM30.883 µL154.416 µL308.833 µL1.544 mL3.088 mL

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Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
Dansyl chloride [5-Dimethylaminonaphthalene-1-sulfonyl chloride] *CAS 605-65-2*33551833000.387

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References


View all 17 references: Citation Explorer
Determination of taurine and hypotaurine in animal tissues by reversed-phase high-performance liquid chromatography after derivatization with dabsyl chloride
Authors: Nakamura H, Ubuka T.
Journal: Adv Exp Med Biol (2003): 221
Dansyl and dabsyl analytical constructs as tools for the accurate estimation of compounds in solid-phase synthesis
Authors: Zaramella A, Conti N, Dal Cin M, Paio A, Seneci P, Gehanne S.
Journal: J Comb Chem (2001): 410
Determination of amphetamine and its metabolite p-hydroxyamphetamine in rat urine by reversed-phase high-performance liquid chromatography after dabsyl derivatization
Authors: Soares ME, Carvalho F, Bastos ML.
Journal: Biomed Chromatogr (2001): 452
Development of a sensitive and quantitative analytical method for 1H-4-substituted imidazole histamine H3-receptor antagonists utilizing high-performance liquid chromatography and dabsyl derivatization
Authors: H, undefined and ley MK, Hirth WW, Phillips JG, Ali SM, Khan A, Fadnis L, Tedford CE.
Journal: J Chromatogr B Biomed Sci Appl (1998): 239
Determination of biogenic amines by RP-HPLC of the dabsyl derivates
Authors: Bockhardt A, Krause I, Klostermeyer H.
Journal: Z Lebensm Unters Forsch (1996): 65
Analysis of phosphorylhydroxyamino acids present in hydrolyzed cell extracts using dabsyl derivatization
Authors: de Witte PA, Cuveele JF, Merlevede WJ, V and enheede JR., undefined
Journal: Anal Biochem (1995): 1
Comparative and optimized dabsyl-amino acid analysis of synthetic phosphopeptides and glycopeptides
Authors: Gorbics L, Urge L, Otvos L, Jr.
Journal: J Chromatogr A (1994): 169
Analysis of physiological amino acids using dabsyl derivatization and reversed-phase liquid chromatography
Authors: Drnevich D, Vary TC.
Journal: J Chromatogr (1993): 137
Reversed-phase high-performance liquid chromatographic analysis of hydroxyproline and proline from collagen by derivatization with dabsyl chloride
Authors: Ikeda M, Sorimachi K, Akimoto K, Yasumura Y.
Journal: J Chromatogr (1993): 133
Fluorogenic bimane substrates with dabsyl group for endopeptidases; chymotrypsin, collagenase and thermolysin
Authors: Sato E, Hattori H, Kanaoka Y.
Journal: J Pharmacobiodyn (1991): 599