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DAX-J2™ IR

DAX-J2™ IR is a new nitric oxide (NO) sensor recently developed by AAT Bioquest. It is a water-soluble fluorogenic reagent that can measure free NO and nitric oxide synthase (NOS) activity in in vivo under physiological conditions. The blocking groups on the DAX-J2 reagent are released to generate the highly fluorescent product upon NO oxidation. DAX-J2 fluorescent product can be detected using the filter set of Cy7® and ICG. DAX-J2 IR has distinct advantages for NO detection than the popular DAF-2 NO probe: 1). It does not require esterase activity for NO detection. DAF-2 requires intracellular esterases to cleave its acetate groups for detecting NO activity. This esterase dependence often complicates the NO detection since esterse activities are affected by cell health and many other factors. 2). DAX-2 product exhibits pH-independent fluorescence while DAF-2 has its fluorescence highly affected by pH. 3). It is more sensitive for detecting NO than DAF-2. 4). It can be used for in vivo imaging of NO.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DAX-J2™ IR to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM98.42 µL492.102 µL984.204 µL4.921 mL9.842 mL
5 mM19.684 µL98.42 µL196.841 µL984.204 µL1.968 mL
10 mM9.842 µL49.21 µL98.42 µL492.102 µL984.204 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)
DAX-J2™ Orange552575
DAX-J2™ Red587609

Citations

View all 2 citations: Citation Explorer
Fluorescent real-time quantitative measurements of intracellular peroxynitrite generation and inhibition
Authors: Luo, Zhen and Zhao, Qin and Liu, Jixiang and Liao, Jinfang and Peng, Ruogu and Xi, Yunting and Diwu, Zhenjun
Journal: Analytical biochemistry (2017): 44--48
Inducible Nitric Oxide Synthase (iNOS) Is a Novel Negative Regulator of Hematopoietic Stem/Progenitor Cell Trafficking
Authors: Adamiak, Mateusz and Abdelbaset-Ismail, Ahmed and Moore, Joseph B and Zhao, J and Abdel-Latif, Ahmed and Wysoczynski, Marcin and Ratajczak, Mariusz Z
Journal: Stem Cell Reviews and Reports (2016): 1--12

References

View all 139 references: Citation Explorer
Pitfalls and limitations in using 4,5-diaminofluorescein for evaluating the influence of polyphenols on nitric oxide release from endothelial cells
Authors: Uhlenhut K, Hogger P.
Journal: Free Radic Biol Med (2012): 2266
Effects of moderate electrical stimulation on reactive species production by primary rat skeletal muscle cells: cross talk between superoxide and nitric oxide production
Authors: Lambertucci RH, Silveira Ldos R, Hirabara SM, Curi R, Sweeney G, Pithon-Curi TC.
Journal: J Cell Physiol (2012): 2511
Improved measurements of intracellular nitric oxide in intact microvessels using 4,5-diaminofluorescein diacetate
Authors: Zhou X, He P.
Journal: Am J Physiol Heart Circ Physiol (2011): H108
Aging negatively affects estrogens-mediated effects on nitric oxide bioavailability by shifting ERalpha/ERbeta balance in female mice
Authors: Novensa L, Novella S, Medina P, Segarra G, Castillo N, Heras M, Hermenegildo C, Dantas AP.
Journal: PLoS One (2011): e25335
Temporal and spatial correlation of platelet-activating factor-induced increases in endothelial [Ca(2)(+)]i, nitric oxide, and gap formation in intact venules
Authors: Zhou X, He P.
Journal: Am J Physiol Heart Circ Physiol (2011): H1788
Page updated on December 6, 2024

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Catalog Number16302
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Physical properties

Molecular weight

1016.05

Solvent

DMSO

Spectral properties

Excitation (nm)

778

Emission (nm)

792

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Fluorescence response of DAX-J2<sup>TM</sup> IR (1 uM) to different reactive oxygen species (1 mM) in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 700/780 nm.
Fluorescence response of DAX-J2<sup>TM</sup> IR (1 uM) to different reactive oxygen species (1 mM) in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 700/780 nm.
Fluorescence response of DAX-J2<sup>TM</sup> IR (1 uM) to different reactive oxygen species (1 mM) in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 700/780 nm.