DEAC [7-Diethylaminocoumarin-3-carboxylic acid] CAS 50995-74-9

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Physical properties

Molecular weight	261.27
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.14
Correction Factor (280 nm)	0.12
Excitation (nm)	411
Emission (nm)	472

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

50995-74-9

Molecular weight

261.27

Correction Factor (260 nm)

0.14

Correction Factor (280 nm)

0.12

Excitation (nm)

411

Emission (nm)

472

7-Diethylaminocoumarin-3-carboxylic acid can be used to create blue-fluorescent bioconjugates. It has quite strong blue fluorescence. When compared with AMCA conjugates, conjugates of the UV-light-excitable 7-dialkylaminocoumarin fluorophore have much longer-wavelength excitation and slightly longer-wavelength emission spectra (~470 nm).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DEAC [7-Diethylaminocoumarin-3-carboxylic acid] *CAS 50995-74-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	382.746 µL	1.914 mL	3.827 mL	19.137 mL	38.275 mL
5 mM	76.549 µL	382.746 µL	765.492 µL	3.827 mL	7.655 mL
10 mM	38.275 µL	191.373 µL	382.746 µL	1.914 mL	3.827 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.14
Correction Factor (280 nm)	0.12
Excitation (nm)	411
Emission (nm)	472

Images

Figure 1. Chemical structure for DEAC [7-Diethylaminocoumarin-3-carboxylic acid] *CAS 50995-74-9*

References

View all 4 references: Citation Explorer

Fluorescent agonists for the Torpedo nicotinic acetylcholine receptor
Authors: Krieger F, Mourot A, Araoz R, Kotzyba-Hibert F, Molgo J, Bamberg E, Goeldner M.
Journal: Chembiochem (2008): 1146

A series of related nucleotide analogues that aids optimization of fluorescence signals in probing the mechanism of P-loop ATPases, such as actomyosin
Authors: Webb MR, Reid GP, Munasinghe VR, Corrie JE.
Journal: Biochemistry (2004): 14463

Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin
Authors: Webb MR, Corrie JE.
Journal: Biophys J (2001): 1562

Glutamate 779, an intramembrane carboxyl, is essential for monovalent cation binding by the Na,K-ATPase
Authors: Arguello JM, Kaplan JH.
Journal: J Biol Chem (1994): 6892