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DEAC [7-Diethylaminocoumarin-3-carboxylic acid] *CAS 50995-74-9*

7-Diethylaminocoumarin-3-carboxylic acid can be used to create blue-fluorescent bioconjugates. It has quite strong blue fluorescence. When compared with AMCA conjugates, conjugates of the UV-light-excitable 7-dialkylaminocoumarin fluorophore have much longer-wavelength excitation and slightly longer-wavelength emission spectra (~470 nm).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DEAC [7-Diethylaminocoumarin-3-carboxylic acid] *CAS 50995-74-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM382.746 µL1.914 mL3.827 mL19.137 mL38.275 mL
5 mM76.549 µL382.746 µL765.492 µL3.827 mL7.655 mL
10 mM38.275 µL191.373 µL382.746 µL1.914 mL3.827 mL

Molarity calculator

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Spectrum

References

View all 4 references: Citation Explorer
Fluorescent agonists for the Torpedo nicotinic acetylcholine receptor
Authors: Krieger F, Mourot A, Araoz R, Kotzyba-Hibert F, Molgo J, Bamberg E, Goeldner M.
Journal: Chembiochem (2008): 1146
A series of related nucleotide analogues that aids optimization of fluorescence signals in probing the mechanism of P-loop ATPases, such as actomyosin
Authors: Webb MR, Reid GP, Munasinghe VR, Corrie JE.
Journal: Biochemistry (2004): 14463
Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin
Authors: Webb MR, Corrie JE.
Journal: Biophys J (2001): 1562
Glutamate 779, an intramembrane carboxyl, is essential for monovalent cation binding by the Na,K-ATPase
Authors: Arguello JM, Kaplan JH.
Journal: J Biol Chem (1994): 6892
Page updated on December 11, 2024

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Physical properties

Molecular weight

261.27

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.14

Correction Factor (280 nm)

0.12

Excitation (nm)

411

Emission (nm)

472

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

50995-74-9
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