dGTP 100 mM PCR Grade

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Physical properties

Molecular weight	507.18
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

2564-35-4

Molecular weight

507.18

2'-Deoxyguanosine-5'-Triphosphate (dGTP) solution is used in DNA synthesis reactions such as PCR, DNA sequencing and molecular cloning techniques. This dATP solution is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of dGTP *100 mM PCR Grade* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	197.169 µL	985.843 µL	1.972 mL	9.858 mL	19.717 mL
5 mM	39.434 µL	197.169 µL	394.337 µL	1.972 mL	3.943 mL
10 mM	19.717 µL	98.584 µL	197.169 µL	985.843 µL	1.972 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Chemical structure for dGTP *100 mM PCR Grade*.

References

View all 50 references: Citation Explorer

DMSO and betaine significantly enhance the PCR amplification of ITS2 DNA barcodes from plants.
Authors: Varadharajan, Bhooma and Parani, Madasamy
Journal: Genome (2021): 165-171

Diagnostic utility of droplet digital PCR to detect TERT promoter mutations among glioblastoma samples using 7-deaza-dGTP.
Authors: Abarna, Rajadurai and Nancy, Rachel and Chacko, Ari and Chacko, Geeta and Pai, Rekha
Journal: Journal of clinical pathology (2021)

Enzymatic Synthesis of Chimeric DNA Oligonucleotides by in Vitro Transcription with dTTP, dCTP, dATP, and 2'-Fluoro Modified dGTP.
Authors: Kapustina, Žana and Jasponė, Aistė and Dubovskaja, Varvara and Mackevičius, Gytis and Lubys, Arvydas
Journal: ACS synthetic biology (2021): 1625-1632

A novel target enrichment strategy in next-generation sequencing through 7-deaza-dGTP-resistant enzymatic digestion.
Authors: Peng, Peng and Xu, Yanjuan and Di Bisceglie, Adrian M and Fan, Xiaofeng
Journal: BMC research notes (2020): 445

Biosynthesis of (deoxy)guanosine-5'-triphosphate by GMP kinase and acetate kinase fixed on the surface of E. coli.
Authors: Yao, Yefeng and Ding, Qingbao and Ou, Ling
Journal: Enzyme and microbial technology (2019): 82-89

Polymerase synthesis of four-base DNA from two stable dimeric nucleotides.
Authors: Mohsen, Michael G and Ji, Debin and Kool, Eric T
Journal: Nucleic acids research (2019): 9495-9501

Use of alternative alkali chlorides in RT and PCR of polynucleotides containing G quadruplex structures.
Authors: Ramos-Alemán, Fabiola and González-Jasso, Eva and Pless, Reynaldo C
Journal: Analytical biochemistry (2018): 43-50

Optimizing Amplification of the GC-Rich TERT Promoter Region Using 7-Deaza-dGTP for Droplet Digital PCR Quantification of TERT Promoter Mutations.
Authors: Colebatch, Andrew J and Witkowski, Tom and Waring, Paul M and McArthur, Grant A and Wong, Stephen Q and Dobrovic, Alexander
Journal: Clinical chemistry (2018): 745-747

Self-Priming Enzymatic Fabrication of Multiply Modified DNA.
Authors: Whitfield, Colette J and Little, Rachel C and Khan, Kasid and Ijiro, Kuniharu and Connolly, Bernard A and Tuite, Eimer M and Pike, Andrew R
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 15267-15274

Cytosine DNA methylation at promoter of non LTR retrotransposon and heat shock protein gene (HSP70) of Entamoeba histolytica and lack of correlation with transcription status.
Authors: Agrahari, Mridula and Gaurav, Amit Kumar and Bhattacharya, Alok and Bhattacharya, Sudha
Journal: Molecular and biochemical parasitology (2017): 21-27