DiIC18(5)-DS [1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine-5,5-disulfonic acid]
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range. This particular near-infrared DiI derivative is an amphiphilic molecule that contains both hydrophilic and lipophilic moiety. It has stronger fluorescence in water than the highly lipophilic DiI analogs.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiIC18(5)-DS [1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine-5,5-disulfonic acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 98.081 µL | 490.403 µL | 980.806 µL | 4.904 mL | 9.808 mL |
5 mM | 19.616 µL | 98.081 µL | 196.161 µL | 980.806 µL | 1.962 mL |
10 mM | 9.808 µL | 49.04 µL | 98.081 µL | 490.403 µL | 980.806 µL |
Molarity calculator
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Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
DiIC12(5)-DS [1,1-Diododecyl-3,3,3,3-tetramethylindodicarbocyanine-5,5-disulfonic acid] | 652 | 670 | - |
DiIC18(3)-DS [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-5,5-disulfonic acid] | 550 | 564 | 148000 |
Citations
View all 44 citations: Citation Explorer
In vivo imaging system for explants analysis—A new approach for assessment of cell transplantation effects in large animal models
Authors: Zarychta-Wi{\'s}niewska, Weronika and Burdzinska, Anna and Zagozdzon, Rados{\l}aw and Dybowski, Bartosz and Butrym, Marta and Gajewski, Zdzis{\l}aw and Paczek, Leszek
Journal: PloS one (2017): e0184588
Authors: Zarychta-Wi{\'s}niewska, Weronika and Burdzinska, Anna and Zagozdzon, Rados{\l}aw and Dybowski, Bartosz and Butrym, Marta and Gajewski, Zdzis{\l}aw and Paczek, Leszek
Journal: PloS one (2017): e0184588
Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure
Authors: Wang, Kun and Li, Yuwen and Zhu, Tiantian and Zhang, Yongting and Li, Wenting and Lin, Wenyu and Li, Jun and Zhu, Chuanlong
Journal: Stem Cell Research & Therapy (2017): 162
Authors: Wang, Kun and Li, Yuwen and Zhu, Tiantian and Zhang, Yongting and Li, Wenting and Lin, Wenyu and Li, Jun and Zhu, Chuanlong
Journal: Stem Cell Research & Therapy (2017): 162
Influence of Particle Geometry on Gastrointestinal Transit and Absorption following Oral Administration
Authors: Li, Dong and Zhuang, Jie and He, Haisheng and Jiang, Sifan and Banerjee, Amrita and Lu, Yi and Wu, Wei and Mitragotri, Samir and Gan, Li and Qi, Jianping
Journal: ACS Applied Materials & Interfaces (2017)
Authors: Li, Dong and Zhuang, Jie and He, Haisheng and Jiang, Sifan and Banerjee, Amrita and Lu, Yi and Wu, Wei and Mitragotri, Samir and Gan, Li and Qi, Jianping
Journal: ACS Applied Materials & Interfaces (2017)
Mesenchymal stem cells increase skin graft survival time and up-regulate PD-L1 expression in splenocytes of mice
Authors: Moravej, Ali and Geramizadeh, Bita and Azarpira, Negar and Zarnani, Amir-Hasan and Yaghobi, Ramin and Kalani, Mehdi and Khosravi, Maryam and Kouhpayeh, Amin and Karimi, Mohammad-Hossein
Journal: Immunology Letters (2017)
Authors: Moravej, Ali and Geramizadeh, Bita and Azarpira, Negar and Zarnani, Amir-Hasan and Yaghobi, Ramin and Kalani, Mehdi and Khosravi, Maryam and Kouhpayeh, Amin and Karimi, Mohammad-Hossein
Journal: Immunology Letters (2017)
Novel approach for the detection of intraperitoneal micrometastasis using an ovarian cancer mouse model
Authors: Alvero, Ayesha B and Kim, Dongin and Lima, Eydis and Sumi, Natalia J and Lee, Jung Seok and Cardenas, Carlos and Pitruzzello, Mary and Silasi, Dan-Arin and Buza, Natalia and Fahmy, Tarek and others, undefined
Journal: Scientific Reports (2017)
Authors: Alvero, Ayesha B and Kim, Dongin and Lima, Eydis and Sumi, Natalia J and Lee, Jung Seok and Cardenas, Carlos and Pitruzzello, Mary and Silasi, Dan-Arin and Buza, Natalia and Fahmy, Tarek and others, undefined
Journal: Scientific Reports (2017)
References
View all 92 references: Citation Explorer
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
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