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FCB
Fluorescein di-beta-D-cellobioside
This non-fluorescent fluorescein substrate generates the bright fluorescein product that has Ex/Em = 492/514 nm, and can be easily detected with a FITC filter set. In general, fluorescein substrates are much more sensitive than coumarin or nitrophenol-based substrates. This fluorescein substrate is used for monitoring cellulase activities. Cellulases are a family of enzymes that include β-glucosidases, endoglucanases and exoglucanases. These enzymes cleave the β-1,4-D-glycosidic bonds that link the glucose units comprising cellulose. In addition to being produced by plants, cellulase activity is found in many fungi and bacteria, including some plant pathogens. Most animal cells are not known to produce cellulase, in which the cellulolytic activity is often carried out by symbionts. The study of cellulase activity has many applications in plant molecular biology, agriculture, and manufacturing. Cellulase is becoming important in the development of alternative fuel sources, as glucose obtained from cellulose hydrolysis is easily fermented into ethanol. Activity of most cellulases can be conveniently monitored using this sensitive fluorescein cellobioside. Upon cleavage, the fluorescent compound, fluorescein, is released and activity measurements are easily obtained in a microtiter plate based assay format.
Principle of InVitroFlow comprising 7 steps. (1) Mutant library generation using a linear DNA template (approx. 6 h), (2) entrapment of mutant cellulase library in (w/o) single emulsions within 0.5&thinsp;h, (3) cell-free expression of mutant library and generation of (w/o/w) emulsions within 4&thinsp;h, (4) sorting of active variants within (w/o/w) emulsions using flow cytometer within 2&thinsp;h, and (5) DNA recovery from (w/o/w) emulsions and PCR gene amplification in 3.5 h. A whole round of InVitroFlow (diversity generation, screening by flow cytometry, amplification) can be completed within 16 h. (6) Cloning and transformation into expression host (2 days), and (7) screening of up to 2,000 beneficial clones in MTP format and characterization of a few variants (7&ndash;12 days). Source: <strong><em>In vitro</em> flow cytometry-based screening platform for cellulase engineering </strong>by K&ouml;rfer et al., <em>Scientific Reports</em>, May 2016.
Principle of InVitroFlow comprising 7 steps. (1) Mutant library generation using a linear DNA template (approx. 6 h), (2) entrapment of mutant cellulase library in (w/o) single emulsions within 0.5&thinsp;h, (3) cell-free expression of mutant library and generation of (w/o/w) emulsions within 4&thinsp;h, (4) sorting of active variants within (w/o/w) emulsions using flow cytometer within 2&thinsp;h, and (5) DNA recovery from (w/o/w) emulsions and PCR gene amplification in 3.5 h. A whole round of InVitroFlow (diversity generation, screening by flow cytometry, amplification) can be completed within 16 h. (6) Cloning and transformation into expression host (2 days), and (7) screening of up to 2,000 beneficial clones in MTP format and characterization of a few variants (7&ndash;12 days). Source: <strong><em>In vitro</em> flow cytometry-based screening platform for cellulase engineering </strong>by K&ouml;rfer et al., <em>Scientific Reports</em>, May 2016.
protocol
Protocol
sds
SDS
COO
COA
CatalogSize
Price
Quantity
140251 mg
Price
 
Physical properties
Molecular weight980.87
SolventDMSO
Spectral properties
Absorbance (nm)487
Correction factor (260 nm)0.32
Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
80000
1
Excitation (nm)498
Emission (nm)517
Quantum yield
0.7900
1
, 
0.95
2
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Documents
Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis
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Page updated on October 25, 2025