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Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)*

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This fluorescein-12-dUTP conjugate can be used as a green fluorescence color with Spectrum Green™ filter set (Spectrum Green™ is the trademark of Vysis).
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Catalog Number17028
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Physical properties
Molecular weight1060.63
SolventWater
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Spectrum
Product family
NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Fluorescein-12-dCTP *1 mM in Tris Buffer (pH 7.5)* 4985178000010.79001, 0.9520.320.35
Fluorescein-12-dATP *1 mM in Tris Buffer (pH 7.5)* 4985178000010.79001, 0.9520.320.35
Fluorescein-12-dGTP *1 mM in Tris Buffer (pH 7.5)* 4985178000010.79001, 0.9520.320.35
References
View all 50 references: Citation Explorer
Effects of Aerobic, Resistance, and Combined Exercise on Markers of Male Reproduction in Healthy Human Subjects: A Randomized Controlled Trial.
Authors: Hajizadeh Maleki, Behzad and Tartibian, Bakhtyar and Chehrazi, Mohammad
Journal: Journal of strength and conditioning research (2019): 1130-1145
Fluorescently Labeled DNA Interacts with Competence and Recombination Proteins and Is Integrated and Expressed Following Natural Transformation of Bacillus subtilis.
Authors: Boonstra, Mirjam and Vesel, Nina and Kuipers, Oscar P
Journal: mBio (2018)
TUNEL labeling with BrdUTP/anti-BrdUTP greatly underestimates the level of sperm DNA fragmentation in semen evaluation.
Authors: Ribeiro, Sofia C and Muratori, Monica and De Geyter, Maria and De Geyter, Christian
Journal: PloS one (2017): e0181802
Role of carvacrol in cardioprotection against myocardial ischemia/reperfusion injury in rats through activation of MAPK/ERK and Akt/eNOS signaling pathways.
Authors: Chen, Yunping and Ba, Lina and Huang, Wei and Liu, Yan and Pan, Hao and Mingyao, E and Shi, Pilong and Wang, Ye and Li, Shuzhi and Qi, Hanping and Sun, Hongli and Cao, Yonggang
Journal: European journal of pharmacology (2017): 90-100
Role of insulin-like growth factor 1 on cross-bred Bos indicus cattle germinal vesicle oocytes exposed to heat shock.
Authors: Lima, Rafaela S and Risolia, Pedro H B and Ispada, Jéssica and Assumpção, Mayra E O A and Visintin, José A and Orlandi, Cássia and Paula-Lopes, Fabíola F
Journal: Reproduction, fertility, and development (2017): 1405-1414