Fluorescein-12-dUTP 1 mM in Tris Buffer (pH 7.5)

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Physical properties

Molecular weight	1060.63
Solvent	Water

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)* *CAS 214154-36-6*

Overview SDS Protocol

Molecular weight

1060.63

Absorbance (nm)

487

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.35

Extinction coefficient (cm ^-1 M ^-1)

80000¹

Excitation (nm)

498

Emission (nm)

517

Quantum yield

0.7900¹, 0.95²

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This fluorescein-12-dUTP conjugate can be used as a green fluorescence color with Spectrum Green™ filter set (Spectrum Green™ is the trademark of Vysis).

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	94.284 µL	471.418 µL	942.836 µL	4.714 mL	9.428 mL
5 mM	18.857 µL	94.284 µL	188.567 µL	942.836 µL	1.886 mL
10 mM	9.428 µL	47.142 µL	94.284 µL	471.418 µL	942.836 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Fluorescein-12-dCTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dATP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dGTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35

Images

Figure 1. Chemical structure for Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)* .

References

View all 50 references: Citation Explorer

Effects of Aerobic, Resistance, and Combined Exercise on Markers of Male Reproduction in Healthy Human Subjects: A Randomized Controlled Trial.
Authors: Hajizadeh Maleki, Behzad and Tartibian, Bakhtyar and Chehrazi, Mohammad
Journal: Journal of strength and conditioning research (2019): 1130-1145

Fluorescently Labeled DNA Interacts with Competence and Recombination Proteins and Is Integrated and Expressed Following Natural Transformation of Bacillus subtilis.
Authors: Boonstra, Mirjam and Vesel, Nina and Kuipers, Oscar P
Journal: mBio (2018)

TUNEL labeling with BrdUTP/anti-BrdUTP greatly underestimates the level of sperm DNA fragmentation in semen evaluation.
Authors: Ribeiro, Sofia C and Muratori, Monica and De Geyter, Maria and De Geyter, Christian
Journal: PloS one (2017): e0181802

Role of carvacrol in cardioprotection against myocardial ischemia/reperfusion injury in rats through activation of MAPK/ERK and Akt/eNOS signaling pathways.
Authors: Chen, Yunping and Ba, Lina and Huang, Wei and Liu, Yan and Pan, Hao and Mingyao, E and Shi, Pilong and Wang, Ye and Li, Shuzhi and Qi, Hanping and Sun, Hongli and Cao, Yonggang
Journal: European journal of pharmacology (2017): 90-100

Role of insulin-like growth factor 1 on cross-bred Bos indicus cattle germinal vesicle oocytes exposed to heat shock.
Authors: Lima, Rafaela S and Risolia, Pedro H B and Ispada, Jéssica and Assumpção, Mayra E O A and Visintin, José A and Orlandi, Cássia and Paula-Lopes, Fabíola F
Journal: Reproduction, fertility, and development (2017): 1405-1414

Semen leukocytes and oxidative-dependent DNA damage of spermatozoa in male partners of subfertile couples with no symptoms of genital tract infection.
Authors: Micillo, A and Vassallo, M R C and Cordeschi, G and D'Andrea, S and Necozione, S and Francavilla, F and Francavilla, S and Barbonetti, A
Journal: Andrology (2016): 808-15

The Sperm Chromatin Structure Assay (SCSA(®)) and other sperm DNA fragmentation tests for evaluation of sperm nuclear DNA integrity as related to fertility.
Authors: Evenson, Donald P
Journal: Animal reproduction science (2016): 56-75

Use of platelet-rich plasma solution applied with composite chondrocutaneous graft technique: an experimental study in rabbit model.
Authors: Sevim, Kamuran Zeynep and Yazar, Memet and Irmak, Fatih and Tekkeşin, Merva Soluk and Yildiz, Kemalettin and Sirvan, Selami Serhat
Journal: Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons (2014): 1407-19

Inhibition of dendritic cell apoptosis by Leishmania mexicana amastigotes.
Authors: Gutiérrez-Kobeh, Laila and de Oyarzabal, Eleané and Argueta, Jesús and Wilkins, Arturo and Salaiza, Norma and Fernández, Edith and López, Oliver and Aguirre, Magdalena and Becker, Ingeborg
Journal: Parasitology research (2013): 1755-62

Correlation between seminal oxidative stress biomarkers and antioxidants with sperm DNA damage in elite athletes and recreationally active men.
Authors: Tartibian, Bakhtyar and Maleki, Behzad Hajizadeh
Journal: Clinical journal of sport medicine : official journal of the Canadian Academy of Sport Medicine (2012): 132-9