AAT Bioquest

Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)*

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Physical properties
Molecular weight1060.63
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Absorbance (nm)
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
0.79001, 0.952
The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This fluorescein-12-dUTP conjugate can be used as a green fluorescence color with Spectrum Green™ filter set (Spectrum Green™ is the trademark of Vysis).


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM94.284 µL471.418 µL942.836 µL4.714 mL9.428 mL
5 mM18.857 µL94.284 µL188.567 µL942.836 µL1.886 mL
10 mM9.428 µL47.142 µL94.284 µL471.418 µL942.836 µL

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Spectral properties

Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Fluorescein-12-dCTP *1 mM in Tris Buffer (pH 7.5)* 4985178000010.79001, 0.9520.320.35
Fluorescein-12-dATP *1 mM in Tris Buffer (pH 7.5)* 4985178000010.79001, 0.9520.320.35
Fluorescein-12-dGTP *1 mM in Tris Buffer (pH 7.5)* 4985178000010.79001, 0.9520.320.35



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