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Fluorescein biotin

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Physical properties
Molecular weight831.01
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Absorbance (nm)
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
0.79001, 0.952
This bifunctional biotin derivative is used for spectrophotometric determination of biotinylation degree. The assay is based on the kinetic analysis of the enhancement of fluorescence of streptavidin/fluorescein biotin complexes in the presence of biotin. The fluorescence enhancement of fluorescein biotin is proportional to the concentration of biotin. Because the assay is amenable for use in small volumes of 5-50 uL or bead-based assays, the detection limits can be extended to the femtomole range. The dynamic aspects allow the assay to be extended to a broader range of applications including its use as an indicator of reagent mixing in laminar-flow assays carried out in microfluidic devices.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein biotin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM120.335 µL601.677 µL1.203 mL6.017 mL12.034 mL
5 mM24.067 µL120.335 µL240.671 µL1.203 mL2.407 mL
10 mM12.034 µL60.168 µL120.335 µL601.677 µL1.203 mL

Molarity calculator

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Spectral properties

Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952



View all 17 references: Citation Explorer
Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein
Authors: Aslan FM, Yu Y, Mohr SC, Cantor CR.
Journal: Proc Natl Acad Sci U S A (2005): 8507
Dynamics of fluorescence dequenching of ostrich-quenched fluorescein biotin: a multifunctional quantitative assay for biotin
Authors: Wu Y, Simons PC, Lopez GP, Sklar LA, Bur and a T., undefined
Journal: Anal Biochem (2005): 221
Preparation and characterisation of antibody modified gelatin nanoparticles as drug carrier system for uptake in lymphocytes
Authors: Balthasar S, Michaelis K, Dinauer N, von Briesen H, Kreuter J, Langer K.
Journal: Biomaterials (2005): 2723
Electrophoretic behavior of streptavidin complexed to a biotinylated probe: a functional screening assay for biotin-binding proteins
Authors: Humbert N, Zocchi A, Ward TR.
Journal: Electrophoresis (2005): 47
Controlled surface functionalization of silica nanospheres by covalent conjugation reactions and preparation of high density streptavidin nanoparticles
Authors: Schiestel T, Brunner H, Tovar GE.
Journal: J Nanosci Nanotechnol (2004): 504
Synthesis of adenosine derivatives as transcription initiators and preparation of 5' fluorescein- and biotin-labeled RNA through one-step in vitro transcription
Authors: Huang F, Wang G, Coleman T, Li N.
Journal: Rna (2003): 1562
A novel intravascular drug delivery method using endothelial biotinylation and avidin-biotin binding
Authors: Hoya K, Guterman LR, Miskolczi L, Hopkins LN.
Journal: Drug Deliv (2001): 215
Organelle pH studies using targeted avidin and fluorescein-biotin
Authors: Wu MM, Llopis J, Adams S, McCaffery JM, Kulomaa MS, Machen TE, Moore HP, Tsien RY.
Journal: Chem Biol (2000): 197
Accurate measurement of avidin and streptavidin in crude biofluids with a new, optimized biotin-fluorescein conjugate
Authors: Kada G, Falk H, Gruber HJ.
Journal: Biochim Biophys Acta (1999): 33
Rapid estimation of avidin and streptavidin by fluorescence quenching or fluorescence polarization
Authors: Kada G, Kaiser K, Falk H, Gruber HJ.
Journal: Biochim Biophys Acta (1999): 44