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FMOC-Asp(TQ2)-OH

FMOC-Asp(TQ2)-OH is a building block for in-sequence Lys labeling by Tide Quencher™ 2 (TQ2). TQ2 is the best quencher that is used to develop a FRET probe with fluorescein or other fluorescent dyes that have the similar fluorescence spectra such as Bodipy FL dyes. It can also be used with rhodamine 6G, 6-TET and 6-JOE derivatives.

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Asp(TQ2)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM131.253 µL656.263 µL1.313 mL6.563 mL13.125 mL
5 mM26.251 µL131.253 µL262.505 µL1.313 mL2.625 mL
10 mM13.125 µL65.626 µL131.253 µL656.263 µL1.313 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExtinction coefficient (cm -1 M -1)Correction Factor (280 nm)Correction Factor (260 nm)
FMOC-Asp(EDANS)-OH *CAS 182253-73-2*59000.107-
FMOC-Asp(TF3)-OH7500010.179-
FMOC-Asp(DABCYL)-OH-0.516-
FMOC-Asp(TQ3)-OH220000.0910.085
FMOC-Glu(TQ2)-OH210000.120.100
FMOC-Lys(TQ2)-OH210000.120.100

Citations

View all 2 citations: Citation Explorer
Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799

References

View all 16 references: Citation Explorer
Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196
Page updated on December 11, 2024

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Catalog Number5206
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Physical properties

Molecular weight

761.89

Solvent

DMF

Spectral properties

Absorbance (nm)

516

Correction Factor (260 nm)

0.100

Correction Factor (280 nm)

0.12

Extinction coefficient (cm -1 M -1)

21000

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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