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AAT Bioquest

FunXite™-1

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight528.84
SolventDMSO
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Molecular weight
528.84
FunXite™-1 is chemically same molecule to FUN® 1 (FUN® 1 is the trademark of ThermoFisher). It is a unique two-color fluorescent viability probe for yeast and fungi. FunXite-1 diffuses into a variety of cells and initially stains the cytoplasm with a diffusely distributed green fluorescence. In some yeasts and fungi, the stain reacts with some cellular substances (e.g., glutathione) to generate distinct vacuolar structures that exhibit a striking red fluorescence, accompanied by a reduction in the green cytoplasmic fluorescence. Formation of the intravacuolar structures requires both plasma membrane integrity and metabolic capability. Dead yeast and fungus cells fluoresce bright yellow-green, with no discernable red structures.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FunXite™-1 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM189.093 µL945.466 µL1.891 mL9.455 mL18.909 mL
5 mM37.819 µL189.093 µL378.186 µL1.891 mL3.782 mL
10 mM18.909 µL94.547 µL189.093 µL945.466 µL1.891 mL

Molarity calculator

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Citations


View all 9 citations: Citation Explorer
Efficacy of propidium iodide and FUN-1 stains for assessing viability in basidiospores of Rhizopogon roseolus
Authors: Fern, undefined and ez-Mir, undefined and a, E., Majada, J., Casares, A.
Journal: Mycologia (2017): 350-358
Use of the vital stain FUN-1 indicates viability of Phytophthora capsici propagules and can be used to predict maximum zoospore production
Authors: Lewis Ivey, M. L., Miller, S. A.
Journal: Mycologia (2014): 362-7
Assessment of FUN-1 vital dye staining: Yeast with a block in the vacuolar sorting pathway have impaired ability to form CIVS when stained with FUN-1 fluorescent dye
Authors: Essary, B. D., Marshall, P. A.
Journal: J Microbiol Methods (2009): 208-12
Saccharomyces cerevisiae samples stained with FUN-1 dye can be stored at -20 degrees C for later observation
Authors: Eggleston, M. D., Marshall, P. A.
Journal: J Microsc (2007): 100-3
Potent synergic effect between ibuprofen and azoles on Candida resulting from blockade of efflux pumps as determined by FUN-1 staining and flow cytometry
Authors: Pina-Vaz, C., Rodrigues, A. G., Costa-de-Oliveira, S., Ricardo, E., Mardh, P. A.
Journal: J Antimicrob Chemother (2005): 678-85
Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells
Authors: Henry-Stanley, M. J., Garni, R. M., Wells, C. L.
Journal: J Microbiol Methods (2004): 289-92
Susceptibility to fluconazole of Candida clinical isolates determined by FUN-1 staining with flow cytometry and epifluorescence microscopy
Authors: Pina-Vaz, C., Sansonetty, F., Rodrigues, A. G., Costa-de-Oliveira, S., Martinez-de-Oliveira, J., Fonseca, A. F.
Journal: J Med Microbiol (2001): 375-82
Development of the FUN-1 family of fluorescent probes for vacuole labeling and viability testing of yeasts
Authors: Millard, P. J., Roth, B. L., Thi, H. P., Yue, S. T., Haugl and , R. P.
Journal: Appl Environ Microbiol (1997): 2897-905
A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells
Authors: Nozawa, Y., Wachi, E., Tominaga, K., Abe, M., Wakasa, H.
Journal: J Pathol (1993): 309-15