FunXite™-1
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 528.84 |
| Solvent | DMSO |
Storage, safety and handling
| Intended use | Research Use Only (RUO) |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
Molecular weight 528.84 |
FunXite™-1 is chemically same molecule to FUN® 1 (FUN® 1 is the trademark of ThermoFisher). It is a unique two-color fluorescent viability probe for yeast and fungi. FunXite-1 diffuses into a variety of cells and initially stains the cytoplasm with a diffusely distributed green fluorescence. In some yeasts and fungi, the stain reacts with some cellular substances (e.g., glutathione) to generate distinct vacuolar structures that exhibit a striking red fluorescence, accompanied by a reduction in the green cytoplasmic fluorescence. Formation of the intravacuolar structures requires both plasma membrane integrity and metabolic capability. Dead yeast and fungus cells fluoresce bright yellow-green, with no discernable red structures.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of FunXite™-1 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 189.093 µL | 945.466 µL | 1.891 mL | 9.455 mL | 18.909 mL |
| 5 mM | 37.819 µL | 189.093 µL | 378.186 µL | 1.891 mL | 3.782 mL |
| 10 mM | 18.909 µL | 94.547 µL | 189.093 µL | 945.466 µL | 1.891 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| Oxazine 1 *CAS 24796-94-9* | 648 | 668 | 123000 | 0.11001 |
| DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7* | 514 | 531 | 1170001 | 0.341 |
| DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* | 491 | 508 | 989001 | 0.521 |
| JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2* | 515 | 530 | 1950001 | - |
| BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole] | 349 | 421 | - | - |
| DiYO™-1 [equivalent to YOYO®-1] | 491 | 508 | 989001 | 0.521 |
| DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution* | 433 | 457 | 920001 | 0.61 |
Images
Figure 1. Chemical structure for FunXite™-1.
Citations
View all 9 citations: Citation Explorer
Efficacy of propidium iodide and FUN-1 stains for assessing viability in basidiospores of Rhizopogon roseolus
Authors: Fern, undefined and ez-Mir, undefined and a, E., Majada, J., Casares, A.
Journal: Mycologia (2017): 350-358
Authors: Fern, undefined and ez-Mir, undefined and a, E., Majada, J., Casares, A.
Journal: Mycologia (2017): 350-358
Use of the vital stain FUN-1 indicates viability of Phytophthora capsici propagules and can be used to predict maximum zoospore production
Authors: Lewis Ivey, M. L., Miller, S. A.
Journal: Mycologia (2014): 362-7
Authors: Lewis Ivey, M. L., Miller, S. A.
Journal: Mycologia (2014): 362-7
Assessment of FUN-1 vital dye staining: Yeast with a block in the vacuolar sorting pathway have impaired ability to form CIVS when stained with FUN-1 fluorescent dye
Authors: Essary, B. D., Marshall, P. A.
Journal: J Microbiol Methods (2009): 208-12
Authors: Essary, B. D., Marshall, P. A.
Journal: J Microbiol Methods (2009): 208-12
Saccharomyces cerevisiae samples stained with FUN-1 dye can be stored at -20 degrees C for later observation
Authors: Eggleston, M. D., Marshall, P. A.
Journal: J Microsc (2007): 100-3
Authors: Eggleston, M. D., Marshall, P. A.
Journal: J Microsc (2007): 100-3
Potent synergic effect between ibuprofen and azoles on Candida resulting from blockade of efflux pumps as determined by FUN-1 staining and flow cytometry
Authors: Pina-Vaz, C., Rodrigues, A. G., Costa-de-Oliveira, S., Ricardo, E., Mardh, P. A.
Journal: J Antimicrob Chemother (2005): 678-85
Authors: Pina-Vaz, C., Rodrigues, A. G., Costa-de-Oliveira, S., Ricardo, E., Mardh, P. A.
Journal: J Antimicrob Chemother (2005): 678-85
Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells
Authors: Henry-Stanley, M. J., Garni, R. M., Wells, C. L.
Journal: J Microbiol Methods (2004): 289-92
Authors: Henry-Stanley, M. J., Garni, R. M., Wells, C. L.
Journal: J Microbiol Methods (2004): 289-92
Susceptibility to fluconazole of Candida clinical isolates determined by FUN-1 staining with flow cytometry and epifluorescence microscopy
Authors: Pina-Vaz, C., Sansonetty, F., Rodrigues, A. G., Costa-de-Oliveira, S., Martinez-de-Oliveira, J., Fonseca, A. F.
Journal: J Med Microbiol (2001): 375-82
Authors: Pina-Vaz, C., Sansonetty, F., Rodrigues, A. G., Costa-de-Oliveira, S., Martinez-de-Oliveira, J., Fonseca, A. F.
Journal: J Med Microbiol (2001): 375-82
Development of the FUN-1 family of fluorescent probes for vacuole labeling and viability testing of yeasts
Authors: Millard, P. J., Roth, B. L., Thi, H. P., Yue, S. T., Haugl and , R. P.
Journal: Appl Environ Microbiol (1997): 2897-905
Authors: Millard, P. J., Roth, B. L., Thi, H. P., Yue, S. T., Haugl and , R. P.
Journal: Appl Environ Microbiol (1997): 2897-905
A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells
Authors: Nozawa, Y., Wachi, E., Tominaga, K., Abe, M., Wakasa, H.
Journal: J Pathol (1993): 309-15
Authors: Nozawa, Y., Wachi, E., Tominaga, K., Abe, M., Wakasa, H.
Journal: J Pathol (1993): 309-15